红眼白水貂FSHβ和NCOA1基因多态性及其与繁殖性状的相关分析

doi:10.16431/j.cnki.1671-7236.2018.02.018

Abstract

Abstract:

This study was performed to investigate the effects of follicle-stimulating hormone beta subunit (FSHβ) and nuclear receptor coactivator 1 (NCOA1) genes on reproductive traits of pink eye White mink. Single nucleotide polymorphisms (SNPs) were detected in 215 individuals by single-strand conformation polymorphism (SSCP)and DNA sequencing, correlation analysis were carried out between candidate gene polymorphisms and reproductive traits using the appropriate model, the effects of different genotypes on reproductive traits, including total number born (TNB) and number born alive (NBA), were analyzed by the least square analysis of SAS 9.4 software. The results showed that two polymorphic sites were found in FSHβ gene, g.1228G > A in intron 1, g.1866 T > C in exon 2. One SNP was found in NCOA1 gene, g.151536T > C in exon 6. B allele of FSHβ gene was predominant allele, A allele of NCOA1 gene was predominant allele; TNB and NBA of AA and AB genotypes in FSHβ g.1228G > A site were extremely significantly higher than BB genotype (P < 0.01); The TNB and NBA for g.1866 > C site genotypes were BB > AB > AA; TNB and NBA of NACOA1 gene AB genotype was extremely significantly higher than AA genotype (P < 0.01); The combing genotypes of 151536T > C-g.1228G > A were significant differences in TNB and NBA (P < 0.05). Therefore, these SNPs could be used for marker assist selection on reproduction traits of the pink eyed White mink.

Key words: pink eyed White mink; reproductive traits; SNP; FSHβ gene; NCOA1 gene

CLC Number:

S813.3

HUO Zishuang, LIU Zongyue, YUE Zhigang, DU Zhiheng, CONG Bo, LIU Linling, YANG Fuhe, SUN Yuzhuo, BAI Xiujuan. Association Analysis Between Polymorphisms of FSHβ, NCOA1 Genes and Its Reproductive Traits in Pink Eyed White Mink[J]. , 2018, 45(2): 429-438.

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Association Analysis Between Polymorphisms of FSHβ, NCOA1 Genes and Its Reproductive Traits in Pink Eyed White Mink

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