还原型谷胱甘肽对氟中毒小鼠附睾的影响

doi:10.16431/j.cnki.1671-7236.2018.01.033

Abstract

Abstract:

To investigate the effect of reduced glutathione (GSH) on the epididymis of mice with fluorosis,50 ICR male mice were randomly divided into five groups:Control group and 4 fluorosis groups,which were given distilled water and distilled water +100 mg/L NaF for 30 d,respectively. Then 3 of 4 fluorosis groups were given 200,400 and 800 mg/kg GSH,respectively. After feeding for 30 d,the epididymis was collected and morphological structure were observed after HE staining,the levels of ROS,MDA and GSH,and the activities of GPx,GSTs and GR in the epididymis were detected. The results showed that compared with the control group,the sperm density,sperm motility,GSH,GPx and GR in 100 mg/L NaF group were all significantly decreased (P<0.05),while that in GSH addition groups were increased than 100 mg/L NaF group,and 800 mg/kg GSH group had the best effect. The rate of teratosperm, ROS and MDA were significantly elevated (P<0.05),while that all decreased in GSH addition groups. Compared with the control group,the epididymis luminal thickness in 100 mg/L NaF group were increased and the number of sperm in the lumen was decreased and the indexes had been restored after GSH supplement,and 800 mg/kg GSH group had the better effect.In summary,after adding GSH,the activity of enzyme in glutathione antioxidant system of mice with fluorosis was increased, epididymis morphology had been restored to a certain extent.

Key words: fluorosis; reduced glutathione (GSH); epididymis; glutathione antioxidant system

CLC Number:

R114

CAO Guodi, LI Sujuan, ZHANG Xiaoqing, SUN Zilong. Effects of Reduced Glutathione on the Epididymis of Mice with Fluorosis[J]. , 2018, 45(1): 256-262.

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Effects of Reduced Glutathione on the Epididymis of Mice with Fluorosis

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