不同桑叶提取物对小鼠脾脏淋巴细胞增殖能力的影响

doi:10.16431/j.cnki.1671-7236.2017.12.029

Abstract

Abstract:

This experiment was aimed to compare the effects of different mulberry leaf extract on spleen lymphocyte proliferation in mice. Mulberry leaf polysaccharides (MLP-1, MLP-2, MLP-3, MLP-4 and MLP-5) were prepared with mulberry leaf aqueous extract (MLAE) by different concentration of alcohol deposit, polysaccharides concentration of all extracts were determined by phenol sulfuric acid method. Five different MLPs and MLAE were as the experimental drug, when polysaccharide concentration was 15.625, 31.25, 62.5, 125 and 250 μg/mL, the changes of spleen B and T lymphocytes proliferation in mice which were stimulated by MLPs and MLAE in single or synergistical stimulation of drugs with LPS and PHA were determined by MTT method. The results showed that when polysaccharide concentration was in the range of 15.625 to 250 μg/mL, MLPs and MLAE in single or synergistical stimulation with LPS and PHA could stimulate the spleen B and T lymphocytes proliferation, MLP-1 at low concentration could stimulate significantly spleen B lymphocyte proliferation remarkably (P<0.05), and MLP-3 and MLP-5 at low concentrations showed strong stimulation ability of T lymphocyte proliferation. When MLAE was in synergistical stimulation with LPS at most concentrations points, the spleen B lymphocytes proliferation in mice were significantly higher than those of the cell control and LPS control groups, which revealed that MLAE could significantly improve the humoral immune function (P<0.05).

Key words: mulberry leaf polysaccharides (MLP); mulberry leaf aqueous extract (MLAE); splenic lymphocytes; proliferation; mice

CLC Number:

S852.2

CHEN Xiao-lan, YANG Hai-feng, QU Jing-wen, LI Ran, JIANG Chun-mao, LU Guang-fu, BO Ruo-nan. Effect of Different Mulberry Leaf Extracts on Spleen Lymphocyte Proliferation in Mice[J]. , 2017, 44(12): 3598-3604.

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Effect of Different Mulberry Leaf Extracts on Spleen Lymphocyte Proliferation in Mice

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