不同毒力传染性法氏囊病病毒衣壳蛋白酵母双杂交诱饵载体的构建及鉴定

Abstract

Abstract: The infectious bursal disease virus (IBDV) strains，Gx (very virulent strain), F9 (intermediate strain) and Gt (attenuated strain) had highly similar genetic background, but their biological characteristics were significantly different. To investigate molecular details of the interaction between various IBDV and their host cells, the genes of VP2 capsid protein of Gx, F9 and Gt were cloned into vector pGBKT7, and the recombinant bait vectors pGBGxVP2, pGBF9VP2 and pGBGtVP2 were constructed. Then the self-activation and toxicity of the bait vectors were tested using the Matchmaker Gold Yeast Two-hybrid System. The results showed that three bait vectors all had no self-activations and toxicities to yeast cells. This research benefited to further investigate the interaction between IBDV and its host cell using the yeast two-hybrid system.

Key words: infectious bursal disease virus (IBDV); capsid protein; yeast two-hybrid; bait vector; self-activation; toxicity

CHEN Yu-ming, ZHANG Li-zhou, REN Xian-gang, GAO Li, QIN Li-ting, GAO Yu-long, WANG Yong-qiang, GAO Hong-lei, WANG Xiao-mei, QI Xiao-le. Construction and Identification of Yeast Two-hybrid Bait Vectors of Capsid Protein of IBDV Different Virulence Strains[J]. .

References

1 祁小乐，高立，邓小芸，等.鸡传染性法氏囊病病毒VP2基因突变对病毒体外复制的影响［J］.畜牧兽医学报，2011，42(11):1577～1583.
2 宋博翠, 陈志宝, 万家余, 等. 酵母双杂交技术筛选朊蛋白 PrP23-231 互作蛋白［J］.中国畜牧兽医, 2010, 37(8): 34～37.
3 高立，祁小乐，高玉龙，等.鸡传染性法氏囊病病毒超强毒株VP2基因突变对细胞嗜性改变的研究［J］.中国预防兽医学报，2010，32(10): 747～751.
4 殷震,刘景华.动物病毒学［M］.第2版.北京:科学出版社,1997.
5 Coates P J, Hall P A. The yeast two-hybrid system for identifying protein-protein interactions［J］.Pathol,2003,199(1):4～7.
6 Li Z, Wang Y, Xue Y, et al. Critical role for voltage-dependent anion channel 2 in infectious bursal disease virus-induced apoptosis in host cells via interaction with VP5［J］. J Virol, 2012, 86(3): 1328～1338.
7 Li Z, Wang Y, Li X, et al. Critical roles of glucocorticoid-induced leucine zipper in infectious bursal disease virus (IBDV)-induced suppression of type Ⅰ interferon expression and enhancement of IBDV growth in host cells via interaction with VP4［J］. J Virol, 2013, 87(2): 1221～1231.
8 Lin T W, Lo C W, Lai S Y, et al. Chicken heat shock protein 90 is a component of the putative cellular receptor complex of infectious bursal disease virus［J］. J Virol, 2007, 81(16): 8730～8741.
9 Ma J, Ptashne M. A new class of yeast transcriptional activators［J］. Cell, 1987, 51(1): 113～119.
10 Müller H, Islam M R, Raue R. Research on infectious bursal disease——The past, the present and the future［J］. Vet Microbiol, 2003, 97(1):153～165.
11 Ogawa M, Yamaguchi T, Setiyono A, et al. Some characteristics of a cellular receptor for virulent infectious bursal disease virus by using flow cytometry［J］. Arch Virol, 1998, 143(12): 2327～2341.
12 Qi X, Gao H, Gao Y, et al. Naturally occurring mutations at residues 253 and 284 in VP2 contribute to the cell tropism and virulence of very virulent infectious bursal disease virus［J］. Antiviral Res, 2009, 84(3): 225～233.
13 Wang X, Fu C, Gao H, et al. Pathogenic antigenic and molecular characterization of the very virulent strain (Gx) of infectious bursal disease virus isolated in China［J］. Agri Sci China, 2003, 2(5): 566～572.
14 Wang X, Zeng X, Gao H, et al. Changes in VP2 gene during the attenuation of very virulent infectious bursal disease virus strain Gx isolated in China［J］. Avian Dis, 2004, 48(1): 77～83.

[1]	JIAO Linlin, CHENG Yuting, WU Qingguo, WU Shuang, WU Zhi, ZHU Shanyuan, QIAN Yingjuan. Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Tembusu Virus Capsid Protein [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2395-2402.
[2]	DAN Yixin, XIANG Hua, ZHANG Huanrong, YANG Lu, REN Yupeng, XU Songwei, HE Honghong, ZHU Jiangjiang. Screening of Host Proteins Interacting with ORFV129 Protein and Cloning and Analysis of C1QBP Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 1-14.
[3]	WANG Yao, YAN Ge, LIU Zhihui, WANG Yu, XIN Quanchao, CAO Xinyue, YU Wenhui, JIANG Xiaowen. Study on the Toxicity and Function Effects of Aristolochic Acid Ⅰ on the Five Viscera of Rats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2757-2767.
[4]	QIU Runhui, GUAN Feihu, TAO Tingting, LI Jia, ZHAO Tianyi, DENG Xingmei, SHI Chao, SUN Zhihua, ZHANG Hui. Subcellular Localization and Screening of Host Interacting Proteins of Brucella Secreted Protein BPE005 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1438-1448.
[5]	ZHANG Wen, QIN Zhibiao, CHENG Jing, YU Meiling, WEI Yingyi, LI Jiang, HU Tingjun. Study on Acute and Subchronic Toxicity of Polygonum hydropiper L. Extract [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1532-1544.
[6]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[7]	LIU Qingqing, WU Peng, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu, XIAO Chencheng. Construction of Recombinant Adenovirus Expressing Porcine Reproductive and Respiratory Syndrome Virus N Protein and Its Immunogenicity Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1024-1031.
[8]	QIAO Chunyu, LIU Jiahe, ZHANG Boxi, HE Yuxi, ZHENG Yuwei, LYU Hongming. Research Progress on Pathogenic Mechanism and Control of Aflatoxin B₁ [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 765-775.
[9]	CUI Shuai, WANG Yang, GUO Xiaoyu, CHEN Shiyu, JIANG Yajun, PANG Zhongbao, HUANG Jianxin, MENG Fanfeng, JIANG Yitong, ZHU Hongfei, JIA Hong. Screening and Identification of the Host Proteins Interacting with African Swine Fever Virus E120R Protein Using Yeast Two-hybrid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4139-4149.
[10]	PAN Chenhao, ZHANG Xin, ZHAO Ruili, JIANG Xuan, JIN Tianming, YU Enyuan, LI Liuan, ZENG Jun, YU Xiaoxue, SONG Qiqi, HU Ye. Construction and Cytotoxicity Detection of Recombinant Epitope Protein DCpep-GSE of Streptococcus suis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2990-3001.
[11]	XIANG Rong, LI Yahui, GAO Yuan, WEI Qilin, SHU Bingyao, LUO Shengjun. Study on Acute Toxicity and 30-day Feeding Test of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz.Water Extract [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2262-2270.
[12]	MENG Weijin, FAN Kuohai, CHANG Xiongxiong, YIN Wei, LI Hongquan, WANG Zhirui. Eukaryotic Expression and Immunogenicity Detection of Fusion Protein of Chicken Infectious Bursal Disease Virus VP2 and Truncated Diphtheria Toxin DT390 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4652-4660.
[13]	XIONG Kuankuan, WU Yingxin, YI Siliang, YANG Lingchen. Mechanism of T-2 Toxin Induced Injury and Oxidative Stress in PK15 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4710-4717.
[14]	ZHAO Junjie, CHEN Kexin, LUAN Yehui, CHENG Linli. Evaluation of Combined Toxicity of Enrofloxacin and Sulfamethazine [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3872-3879.
[15]	GUO Yuli, LIU Junping, SUN Di, DONG Zhiying, WANG Peng, LIU Chenggong. Study on Toxicity and Safety Evaluation of Qinzhu Antai Powder [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3880-3888.

Construction and Identification of Yeast Two-hybrid Bait Vectors of Capsid Protein of IBDV Different Virulence Strains

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments