不同毒力传染性法氏囊病病毒衣壳蛋白酵母双杂交诱饵载体的构建及鉴定

Abstract

Abstract: The infectious bursal disease virus (IBDV) strains，Gx (very virulent strain), F9 (intermediate strain) and Gt (attenuated strain) had highly similar genetic background, but their biological characteristics were significantly different. To investigate molecular details of the interaction between various IBDV and their host cells, the genes of VP2 capsid protein of Gx, F9 and Gt were cloned into vector pGBKT7, and the recombinant bait vectors pGBGxVP2, pGBF9VP2 and pGBGtVP2 were constructed. Then the self-activation and toxicity of the bait vectors were tested using the Matchmaker Gold Yeast Two-hybrid System. The results showed that three bait vectors all had no self-activations and toxicities to yeast cells. This research benefited to further investigate the interaction between IBDV and its host cell using the yeast two-hybrid system.

Key words: infectious bursal disease virus (IBDV); capsid protein; yeast two-hybrid; bait vector; self-activation; toxicity

CHEN Yu-ming, ZHANG Li-zhou, REN Xian-gang, GAO Li, QIN Li-ting, GAO Yu-long, WANG Yong-qiang, GAO Hong-lei, WANG Xiao-mei, QI Xiao-le. Construction and Identification of Yeast Two-hybrid Bait Vectors of Capsid Protein of IBDV Different Virulence Strains[J]. China Animal Husbandry & Veterinary Medicine.

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Construction and Identification of Yeast Two-hybrid Bait Vectors of Capsid Protein of IBDV Different Virulence Strains

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