地菍总黄酮对高脂饮食联合链脲佐菌素诱导糖尿病肾病小鼠脂质过氧化的影响

doi:10.16431/j.cnki.1671-7236.2025.01.033

Abstract

Abstract: 【Objective】 This study was aimed to investigate the effect of total flavonoids from Melastoma dodecandrum Lour.(TFMD) on lipid peroxidation in diabetic nephropathy (DN) mice,and explore the medicinal value of TFMD in renal function protection and anti-lipid peroxidation,so as to provide experimental basis for the clinical application of Yao medicine Melastoma dodecandrum Lour. 【Method】 Ninety SPF male C57BL/6J mice were randomly divided into blank group (n＝10) and model group (n=80).Mice in blank group were given normal diet,and mice in model group were given high-fat diet for 6 weeks,after fasting for 12 h,mice in model group were intraperitoneally injected with streptozotocin (STZ) 0.05 g/kg,and mice in blank group were injected with the same dose of sodium citrate buffer.After 5 days of continuous injection,fasting blood glucose (FBG) was measured at 72 h and 1 week after the last injection.Mice with FBG≥16.7 mmol/L for two consecutive times were randomly divided into DN model group (high-fat diet),metformin group (high-fat diet＋0.5 g/kg metformin),TFMD high-dose group (high-fat diet＋1.2 g/kg TFMD),TFMD middle-dose group (high-fat diet＋0.8 g/kg TFMD) and TFMD low-dose group (high-fat diet＋0.6 g/kg TFMD),10 mice in each group.After 10 weeks of administration,the general physiological status of mice was observed during administration,and FBG was detected weekly.After 10 weeks of administration,24 h urine was collected,FBG of mice in each group was detected by cutting tail,and the kidney index were calculated. The levels of urine protein (UP) and microalbumin (MAU),serum urea nitrogen (BUN) and serum creatinine (SCr),and activities of glutathione (GSH) and superoxide dismutase (SOD) and levels of malondialdehyde (MDA) in renal tissue were detected.The expression of Nephrin,podocin,PTGS2 and ACSL4 proteins in renal tissue of mice in each group was detected by Western blotting.HE and PAS staining sections of kidney tissues of mice in each group were made and pathological changes were observed. 【Result】 Compared with blank group,the body weight,GSH and SOD activities of mice in DN model group were extremely significantly decreased (P＜0.01),FBG,kidney index,the levels of MAU,UP,SCr,BUN and MDA were extremely significantly increased (P＜0.01),the expression of Nephrin and podocin proteins in renal tissue was extremely significantly decreased (P＜0.01),the expression of PTGS2 and ACSL4 were significantly increased (P＜0.05),and severe damage was observed in renal tissue under light microscope,indicating that the DN model was constructed successfully.TFMD could improve the weight loss of DN mice,significantly reduce the kidney index and FBG (P＜0.05 or P＜0.01),reduce the levels of UP,MAU,SCr and BUN in DN mice (P＜0.01),and increase the levels of Nephrin and podocin in renal tissue (P＜0.05).The damage of kidney tissue in TFMD group was recovered to different degrees.TFMD also increased the levels of GSH and SOD in DN kidney tissue (P＜0.05 or P＜0.01),decreased the level of MDA (P＜0.05 or P＜0.01),down-regulated the expression of lipid peroxidation-related proteins PTGS2 and ACSL4 in kidney tissue (P＜0.05 or P＜0.01),improved the body’s antioxidant capacity and slowed down the body’s lipid peroxidation and its product accumulation. 【Conclusion】 TFMD could significantly delay the occurrence and development of DN induced by high-fat diet combined with STZ,and its mechanism was related to the regulation of lipid peroxidation and oxidative stress,and the regulation of PTGS2/ACSL4 signaling pathway.

Key words: total flavonoids from Melastoma dodecandrum Lour.; diabetic nephropathy; lipid peroxidation; oxidative stress; lipotoxicity

CLC Number:

S853.74

TANG Yufei, MO Yeyun, LI Xiaoxiao, YANG Qiuli, LIN Huilyu, HUANG Haifang, LIN Meiying, LI Li. Effect of Total Flavonoids from Melastoma dodecandrum Lour.on Lipid Peroxidation in Diabetic Nephropathy Mice Induced by High-fat Diet Combined with Streptozocin[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 364-375.

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Effect of Total Flavonoids from Melastoma dodecandrum Lour.on Lipid Peroxidation in Diabetic Nephropathy Mice Induced by High-fat Diet Combined with Streptozocin

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