China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (8): 3391-3402.doi: 10.16431/j.cnki.1671-7236.2023.08.037

• Basic Veterinary Medicine • Previous Articles    

Mechanism of Lonicerae Japonicae Flos Alleviating Oxidative Stress in Cattle Based on Network Pharmacology and Molecular Docking

LIU Weiwei1, ZHANG Yuxin1,2, ZHOU Weiwei1, DAI Xiaofeng1, FU Hongyun3, LUO Wenjie4, LI Xiumei1   

  1. 1. Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. School of Pharmacy, Harbin University of Commerce, Harbin 150028, China;
    3. Qinghai Forage Technology Promotion Station, Xining 810099, China;
    4. Qinghai Provincial Animal Husbandry Station, Xining 810008, China
  • Received:2023-02-06 Published:2023-07-27

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Abstract: 【Objective】 The aim of this study was to analyze and predict the potential active ingredients and targets of Lonicerae Japonicae Flos to alleviate oxidative stress in cattle and explore its possible mechanism of action.【Method】 The active ingredients of Lonicerae Japonicae Flos were collected using Traditional Chinese Medicine Database and Analysis Platform (TCMSP) and literature search,and the targets corresponding to the active ingredients were identified using TCMSP and SwissTargetPrediction databases,and oxidative stress in cattle-related genes were collected in the gene platform of the NCBI database.The STRING database and Cytoscape 3.8.2 software were used to construct and analyze the protein-protein interaction network of Lonicerae Japonicae Flos and oxidative stress in cattle.GO function and KEGG pathway enrichment analysis of the intersection targets were performed using the DAVID database.Molecular docking was performed by AutoDock software to verify the binding activity of key active ingredients to the core targets.【Result】 There were 37 active ingredients of Lonicerae Japonicae Flos,which were involved in 37 targets related to oxidative stress in cattle.Among them,quercetin,kaempferol,luteolin,rutin and chryseriol were the key active ingredients of Lonicerae Japonicae Flos to alleviate oxidative stress in cattle,and tumor necrosis factor (TNF),prostaglandin-endoperoxide synthase 2 (PTGS2),catenin beta-1 (CTNNB1),serum albumin (ALB),caspase 3 (CASP3),catalase (CAT) and tumor protein p53 (TP53) were the core targets to alleviate oxidative stress in cattle.GO functional enrichment and KEGG pathway analysis showed that the targets were involved in biological process, cell component and molecular function,and were involved in multiple metabolic pathways such as HIF-1 signaling pathway,peroxisome,and MAPK signaling pathway.Molecular docking confirmed that quercetin,kaempferol and luteolin,the key active ingredients of honeysuckle,have good binding activities to the common core targets TNF,PTGS2,CTNNB1,ALB,CASP3,CAT and TP53.【Conclusion】 The results of the study revealed the potential active ingredients of Lonicerae Japonicae Flos to alleviate oxidative stress in cattle were quercetin,kaempferol,luteolin,etc.,and the potential action targets were TNF,PTGS2,CTNNB1,etc.,providing theoretical references for the development and subsequent research of Lonicerae Japonicae Flos as a functional feed additive.

Key words: Lonicerae Japonicae Flos; cattle; oxidative stress; network pharmacology; molecular docking

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