海南黑山羊小分子热休克蛋白9 cDNA克隆及其生物信息学分析

Abstract

Abstract: RACE and RT-PCR were used to clone the full length of small heat shock protein 9 coding sequence from Hainan Black goat testis and the bioinformatics analysis was performed. The results showed that the full length of Hspb9 cDNA was 549 bp, including 471 bp in CDS which encoded a protein of 157 amino acids. The sequence was submitted to GenBank,Accession No. was JX088726. It had no trans-membrane region and signal peptide of the Hspb9 protein. There were several phosphpryiation sites in the sequence. The protein contained 15.28% of α-helix,30.57% of β-sheet, and 54.15% of coil. An ubiquitin domain (UBQ) was also predicted. The Clustel W alignment results showed that goat Hspb9 had a close relationship with sheep and cattle.

Key words: small heat shock protein 9; Hainan Black goat; cDNA cloning; bioinformatics analysis

CLC Number:

Q785

SHI Li-guang, CAO Ting, HOU Guan-yu, ZHOU Han-lin, HUANG Xian-zhou, XUN Wen-juan. Cloning and Bioinformatics Prediction of Small Heat Shock Protein cDNA in Hainan Black Goat[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(10): 56-60.

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Cloning and Bioinformatics Prediction of Small Heat Shock Protein cDNA in Hainan Black Goat

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