猪锚蛋白重复序列和SOCS盒蛋白基因4与生长因子受体结合蛋白基因10的克隆及印迹状态分析

Abstract

Abstract: In this study, by using a single nucleotide polymorphism-based method, 1350 bp cDNA sequence of porcine ankyrin repeat and SOCS box containing protein 4 (Asb4) and 1811 bp cDNA sequence of porcine growth factor receptor-bound protein 10 (Grb10) was obtained. Then we identified their imprinting status in ten tissues. Imprinting analysis showed that both alleles of Asb4 were expressed in all the tested tissues. Grb10 was paternally expressed in tongue, kidney, stomach, intestine and brain, while biallelically expressed in the others. We also used quantitative Real-time PCR to assess the expression of Grb10 in these tissues. The expression level of Grb10 was significantly different among tissues, which was the highest in lung and lowest in both small intestine and brain. These results primarily indicated that Grb10 probably was a porcine paternally expressed gene, but Asb4 was not imprinted in swine.

Key words: pig; ankyrin repeat and SOCS box containing protein 4; growth factor receptor-bound protein 10; single nucleotide polymorphism; imprinting gene

CLC Number:

S828

ZHOU Yang, ZHU Jiang, JIAO Ming-xia, WANG Jia-qiang, KONG Qing-ran, LIU Zhong-hua. Cloning and Imprinting Analysis of Ankyrin Repeat and SOCS Box Containing Protein 4 and Growth Factor Receptor-bound Protein 10 in Swine[J]. , 2013, 40(8): 1-7.

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Cloning and Imprinting Analysis of Ankyrin Repeat and SOCS Box Containing Protein 4 and Growth Factor Receptor-bound Protein 10 in Swine

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