猪圆环病毒2型灭活疫苗种毒株的筛选

Abstract

Abstract: To select porcine circovirus type 2 strain to meet the requirements for producing porcine circovirus type 2 inactivated vaccine, the efficacy and immunogenicity of 8 porcine circovirus type 2 strains from 8 regions of Henan province were assessed by measurement the antibody level of mice immunized with inactivated oil adjuvant vaccine which was manufactured with virus stock solution and diluted solution of same titer (4.233×10⁴ copies/μL). The antibody levels were induced by vaccines manufactured with 8# and 12# strains stock solution were higher and lasted longer than other strains and reference vaccines. The antibody titers at 1 week after the secondary immunization were above required minimum protective level of 1∶800 according to national quality standards of porcine circovirus type 2 inactivated vaccine. These results indicated that porcine circovirus type 2 of 8# and 12# strains could propagate well and had good immunogenicity and therefore could be chosen as candidate vaccine strains. This study enriched the resources of porcine circovirus vaccine strains and provided basis for developing high-quality porcine circovirus vaccine.

Key words: porcine circovirus type 2; inactivated vaccine; candidate vaccine strains

CLC Number:

S852.65^＋9.2

GAO Jin-liang, YANG Xia, CHEN Lu, LI Wei-hao, CHANG Hong-tao, ZHAO Jun, WANG Xin-wei, LIU Hong-ying, YAO Hui-xia, WANG Chuan-qing. Screening of Porcine Circovirus Type 2 Inactivated Vaccine Candidate Strain[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(4): 221-224.

References

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Screening of Porcine Circovirus Type 2 Inactivated Vaccine Candidate Strain

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