Abstract: It was isolated from blood, liver, brain, spleen of mortality pigeons in a pigeon farm in the pearl river delta region, inoculated on MacConkey medium and xylose, lysine, deoxycholate medium, after bacterial purification, microscopic examination, biochemical test, PCR test and drug sensitivity test, results showed that, 8 strains of Salmonella were isolated from 30 samples, the isolation rate was 26.7%, 10 strains of E.coli were isolated, the rate was 33.3%, 6 pigeons were infected Salmonella and E.coli, mixed infection rate was 20.0%. By virus isolation and PCR testing, results showed that 5 avian paramyxovirus of the 6 samples were isolated, the isolation rate of 83.3%. The incidence of death was caused by mixed infection of pigeon paramyxovirus, Salmonella and E.coli.

Key words: pigeon; paramyxovirus Ⅰ type; Salmonella; E.coli; mixed infection