和牛OPU卵母细胞体外受精后胚胎发育潜能研究

doi:10.16431/j.cnki.1671-7236.2025.02.023

Abstract

Abstract: 【Objective】 Oocytes collected by ovum pickup (OPU) in Japanese Black cattle (Wagyu) were matured in vitro (IVM) in medium supplemented with different sources of follicle-stimulating hormone (FSH) and luteinizing hormone (LH),and the in vitro and in vivo developmental potentials were studied after in vitro fertilization (IVF) and in vitro culture (IVC). 【Method】 According to the 3×2 experimental design,OPU oocytes were matured in 6 groups of IVM media in combinations with 3 FSH (NIH-FSH,NB-FSH and CA-FSH,0.13 IU/mL) and 2 LH (NIH-LH and NB-LH,23 IU/mL) for 22-24 h.The blastocyst developmental rate was compared after IVF and IVC.The pregnancy rate was examined by ultrasound 40 days after embryo transfer (ET) to the synchronized recipients. 【Result】 The best combination was supplementing 0.13 IU/mL NIH-FSH and 23 IU/mL NIH-LH in IVM medium,and the blastocyst development rate was up to 47.3%.The pregnancy rate of blastocysts (62.6%-64.0%) derived from OPU oocytes cultured for 6 days(IVC 6D)post IVM and IVF was not significantly different from that of in vivo embryos (P＞0.05),but significantly higher than that of embryos cultured for 7 days (IVC 7D) (44.2%-47.1%) (P＜0.05).The pregnancy rate of blastocysts transferred into heifer recipients was as high as 67.1%,and open recipients after pervious ET cycle could be used for next ET treatment,but the pregnancy rate was significantly lower when ET was repeated 4 times compared with 0 and 1 times (P＜0.05). 【Conclusion】 This study demonstrated that different sources of FSH and LH in combinations in IVM medium had a significant effect on the embryonic development potential of OPU-IVF oocytes.The pregnancy rate of blastocysts from IVC 6D was significantly higher than that from IVC 7D.The optimized OPU-IVF-ET system could effectively cultivate excellent Wagyu breed and rapidly expand its production population.

Key words: Wagyu; ovum pickup; in vitro fertilization; embryo transfer; pregnancy

CLC Number:

S814.8

XU Guangyong, LIU Zhentian, CHEN Xiangfei, ZHANG Meijie, DU Tao, ZHANG Junbo, LIU Zhihui, XING Xuesong, DU Fuliang. Study on Embryonic Developmental Potential of Embryos Derived from Ovum Pickup Oocytes and Fertilization in vitro in Wagyu[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 730-739.

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Study on Embryonic Developmental Potential of Embryos Derived from Ovum Pickup Oocytes and Fertilization in vitro in Wagyu

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