硬脂酸对奶山羊乳腺上皮细胞活力和乳脂合成的影响

doi:10.16431/j.cnki.1671-7236.2025.02.008

Abstract

Abstract: 【Objective】 The aim of this study was to explore the effects of different concentrations of stearic acid on the viability and milk fat synthesis of goat mammary epithelial cells (GMECs). 【Method】 Firstly,GMECs were cultured in vitro and treated with different doses of stearic acid (0,12.5,25,50,100 and 200 μmol/L) for 36 h,the effect on cell viability was detected by CCK-8 method,and the effect on cell triglyceride content was detected by cell triglyceride detection kit.The maximum concentration of stearic acid that had no inhibitory effect on cell viability was chose to treat cells,the effect of stearic acid on lipid droplet accumulation of GMECs were detected by oil red O staining.The mRNA expression levels of genes related to milk fat synthesis were detected by Real-time quantitative PCR. 【Result】 When the concentration of stearic acid was in the range of 0-100 μmol/L,it had no significant effect on cell viability (P>0.05),but when the concentration of stearic acid was 200 μmol/L,the activity of GMECs was significantly lower than that of the control group (P＜0.05).Compared with the control group,the triglyceride content and lipid droplet accumulation in GMECs of the 100 μmol/L stearic acid group were significantly or extremely significantly increased (P＜0.05 or P＜0.01).The Real-time quantitative PCR results showed that compared with the control group,after treatment with 100 μ mol/L stearic acid,the mRNA expression levels of cluster of differentiation 36 (CD36) and diacylglycerol O-acyltransferase homolog 1 (DGAT1) were extremely significantly or significantly increased (P＜0.01 or P＜0.05),The mRNA expression levels of heart-type fatty acid binding protein 3 (FABP3),stearoyl-CoA desaturase 1 (SCD1),adipose triglyceride lipase (ATGL) and forkhead box transcription factor O1 (FoxO1) were extremely significantly or significantly reduced (P＜0.01 or P＜0.05),while the mRNA expression levels of fatty acid synthase (FASN) and sterol-regulatory element binding protein 1 (SREBF1) genes were not significantly affected (P＞0.05). 【Conclusion】 100 μmol/L stearic acid could promote the synthesis of triglycerides and lipid droplet secretion in GMECs by regulating the expression of genes related to milk fat metabolism.This study provided a reference for exogenous addition of long-chain fatty acids to regulate milk fat synthesis and metabolism,which was of great significance for further improving the lactation performance and milk quality of dairy goats.

Key words: dairy goat; stearic acid; mammary epithelial cells; milk fat synthesis

CLC Number:

S827

LI Jun, YU Qianqian, LI Jialin, LI Zhongying, HAN Haoyuan, SHI Huibin, LIU Kun, ZHANG Hao, QUAN Kai. Effects of Stearic Acid on Cell Viability and Milk Fat Synthesis in Goat Mammary Epithelial Cells[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 574-581.

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Effects of Stearic Acid on Cell Viability and Milk Fat Synthesis in Goat Mammary Epithelial Cells

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