血清4型禽腺病毒感染LMH细胞后干扰素刺激基因在转录水平表达量的动态变化

doi:10.16431/j.cnki.1671-7236.2024.05.029

Abstract

Abstract: 【Objective】 This study was aimed to investigate the changes of interferon (IFN) and interferon-stimulating genes (ISGs) expression in chicken Leghorn male hepatoma (LMH) infected with Fowl adenovirus serotype-4 (FAdV-4),and provide reference basis for the study of the interaction between FAdV-4 and ISGs.【Method】 The experiment was conducted by infecting LMH cells with FAdV-4,observing the cellular lesions and collecting cell samples at 0,12,24,36,48,60,72,84 and 96 h post-infection,and the dynamic change patterns of the expression of IFN-α and IFN-β and ISGs genes at the transcriptional level at different time points after infection were detected by Real-time quantitative PCR.【Result】 The results showed that after FAdV-4 infection of LMH cells,LMH cells showed typical cytopathic lesions at 48 h.It was detected that FAdV-4 proliferated rapidly at 12 h,and peaked at 60 h. Compared with control group,the expression of IFN-α at the transcriptional level was significantly down-regulated after infection (P＜0.05).Compared with control group,the expression of IFN-β was significantly down-regulated at 12 and 24 h after infection (P＜0.05),and then rapidly and significantly up-regulated to the peak at 36 h (P＜0.05),and remained at a high level at 48 h.After that,the expression declined and stabilized,but was still significantly up-regulated compared with control group (P＜0.05).The expressions of ISG12,IFIT5 and DDIT4 genes did not change much in the early stage of infection,and reached the peak at 96 h after infection (P<0.05).ZFP313,IFITM3 and Viperin genes did not change much in the early stage of infection,and then rapidly and up-regulated to peak at 36 h (P＜0.05),and remained at a high level at 48 h.After that,the expression declined,but was still significantly up-regulated compared with control group(P＜0.05).CD47,OAS and PKR genes showed down-regulated expression compared with control group.【Conclusion】 After FAdV-4 infection of LMH cells,IFN and a variety of ISGs showed regular changes at the transcriptional level,which were associated with the replication of the virus in LMH cells,suggesting that these natural immune factors might play an important role in the anti-FAdV-4 viral response.

Key words: Fowl adenovirus serotype-4 (FAdV-4); LMH cells; interferon; interferon-stimulated genes; transcription

CLC Number:

S852.65

WAN Lijun, WANG Sheng, XIE Zhixun, REN Hongyu, XIE Liji, FAN Qing, LUO Sisi, LI Meng, ZHANG Yanfang, ZENG Tingting, HUANG Jiaoling, ZHANG Minxiu, XIE Zhiqin, LI Xiaofeng, WEI You. Dynamic Changes in Expression of Interferon-stimulated Genes at the Transcriptional Level After Infection with Fowl Adenovirus Serotype-4 in LMH cells[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 2081-2090.

References

[1] MAZAHERI A,PRUSAS C,VOSS M,et al.Some strains of serotype 4 Fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens[J].Avian Pathology,1998,27(3):269-276.
[2] RASHID F,XIE Z,ZHANG L,et al.Genetic characterization of Fowl aviadenovirus 4 isolates from Guangxi,China,during 2017-2019[J].Poultry Science,2020,99(9):4166-4173.
[3] CHEN H,DOU Y,ZHENG X,et al.Hydropericardium hepatitis syndrome emerged in cherry valley ducks in China[J].Transboundary and Emerging Diseases,2017,64(4):1262-1267.
[4] CHANGJING L,HAIYING L,DONGDONG W,et al.Characterization of Fowl adenoviruses isolated between 2007 and 2014 in China[J].Veterinary Microbiology,2016,197:62-67.
[5] XUE X,YANG Q,WU M J,et al.Genomic and pathologic characterization of the first FAdV-C serotype 4 isolate from black-necked Crane[J].Viruses,2023,15(8):1653.
[6] GANESH K,RAGHAVAN R.Hydropericardium hepatitis syndrome of broiler poultry:Current status of research[J].Research in Veterinary Science,2000,68(3):201-206.
[7] YEO J I,LEE R,KIM H,et al.Genetic modification regulates pathogenicity of a Fowl adenovirus 4 strain after cell line adaptation (genetic mutation in FAdV-4 lowered pathogenicity)[J].Heliyon,2023,9(9):e19860.
[8] GRGIC H,POLJAK Z,SHARIF S,et al.Pathogenicity and cytokine gene expression pattern of a serotype 4 Fowl adenovirus isolate[J].PLoS One,2013,8(10):e77601.
[9] LI P H,ZHENG P P,ZHANG T F,et al.Fowl adenovirus serotype 4:Epidemiology,pathogenesis,diagnostic detection,and vaccine strategies[J].Poultry Science,2017,96(8):2630-2640.
[10] NIU Y,SUN Q,SHI Y,et al.Immunosuppressive potential of Fowl adenovirus serotype 4[J].Poultry Science,2019,98(9):3514-3522.
[11] NIU Y,SUN Q,LIU X,et al.Mechanism of Fowl adenovirus serotype 4-induced heart damage and formation of pericardial effusion[J].Poultry Science,2019,98(3):1134-1145.
[12] 栾勇娇,谢芝勋,王盛,等.血清4型禽腺病毒广西分离株GX2019-010全基因组测序与分析[J].中国畜牧兽医,2020,47(12):3793-3804. LUAN Y J,XIE Z X,WANG S,et al.Whole-genome sequencing analysis of Fowl adenovirus serotype 4 strain GX2019-010 isolated from Guangxi[J].China Animal Husbandry & Veterinary Medicine,2020,47(12):3793-3804.(in Chinese)
[13] 赵玉杰,刘建勋,陈田田,等.血清4型禽腺病毒W株的分离鉴定和全基因组序列分析[J].中国畜牧兽医,2019,46(7):2059-2068. ZHAO Y J,LIU J X,CHEN T T,et al.Isolation,identification and complete genome sequence analysis of Fowl adenovirus genotype 4 W strain[J].China Animal Husbandry & Veterinary Medicine,2019,46(7):2059-2068.(in Chinese)
[14] 尹德晶,张云丹,岳筠,等.禽腺病毒血清4型贵州株全基因序列分析[J].中国畜牧兽医,2021,48(1):44-54. YIN D J,ZHANG Y D,YUN Y,et al.Whole genomic sequencing analysis of Fowl adenovirus serotype 4 from Guizhou[J]. China Animal Husbandry & Veterinary Medicine,2021,48(1):44-54.(in Chinese)
[15] 白思宇,杨倩,仇华吉.干扰素刺激基因的抗病毒机制[J].微生物学报,2018,58(3):361-371. BAI S Y,YANG Q,QIU H J.Antiviral mechanisms of interferon-stimulated genes[J].Acta Microbiologica Sinica, 2018,58(3):361-371.(in Chinese)
[16] 宁菲,胡小玉.干扰素诱导基因的表达调控及作用机制[J].中国免疫学杂志,2019,35(12):1409-1415. NING F,HU X Y.Transcriptional regulation and function of interferon-stimulated genes[J].Chinese Journal of Immunology,2019,35(12):1409-1415.(in Chinese)
[17] 石勇丽,谢芝勋,罗思思,等.血清4型禽腺病毒感染LMH细胞中Toll样受体mRNA转录水平的动态变化[J].中国兽医科学,2022,52(1):85-92. SHI Y L,XIE Z X,LUO S S,et al.Dynamic changes of mRNA transcription levels of Toll-like receptors of Fowl adenovirus serotype 4 infected LMH cells[J].Chinese Veterinary Science,2022,52(1):85-92.(in Chinese)
[18] 张蕾.FAdV-4广西分离株全基因测序分析及其感染CEL和SPF鸡对细胞因子mRNA转录水平影响的研究[D].南宁:广西大学,2020. ZHANG L.Sequence analysis of the whole genome and pathogenicity analysis of Fowl adenovirus serotype 4 from Guangxi[D].Nanning:Guangxi University,2020.(in Chinese)
[19] WU N,YANG B,WEN B,et al.Pathogenicity and immune responses in specific-pathogen-free chickens during Fowl adenovirus serotype 4 infection[J].Avian Diseases,2020,64(3):315-323.
[20] ZHAO W,LI X,LI H,et al.Fowl adenoviruse-4 infection induces strong innate immune responses in chicken[J].Comparative Immunology and Microbiology and Infectious Diseases,2020,68:101404.
[21] SCHNEIDER W M,CHEVILLOTTE M D,RICE C M.Interferon-stimulated genes:A complex web of host defenses[J].Annual Review of Immunology,2014,32(1):513-545.
[22] BORDEN E C,SEN G C,UZE G,et al.Interferons at age 50:Past,current and future impact on biomedicine[J].Nature Reviews Drug Discovery,2007,6(12):975-990.
[23] LI X,JIA Y,LIU H,et al.High level expression of ISG12(1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication[J].Veterinary Microbiology,2018,228:147-156.
[24] WANG S,WAN L,REN H,et al.Screening of interferon-stimulated genes against Avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5[J].Frontiers in Microbiology,2022,13:998505.
[25] KO J H,JIN H K,ASANO A,et al.Polymorphisms and the differential antiviral activity of the chicken Mx gene[J].Genome Research,2002,12(4):595-601.
[26] 谭宗成.鸡α干扰素基因及其下游基因Mx的研究[D].汕头:汕头大学,2008. TAN Z C.Study on chicken interferon-α gene and its downstream gene Mx[D].Shantou:Shantou University,2008.(in Chinese)
[27] RICHARDSON R B,OHLSON M B,EITSON J L,et al.A CRISPR screen identifies IFI6 as an ER-resident interferon effector that blocks Flavivirus replication[J].Nature Microbiology,2018,3(11):1214-1223.
[28] 朱明君.鸡CCCH型锌指抗病毒蛋白抗J亚群禽白血病病毒机制研究[D].泰安:山东农业大学,2020. ZHU M J.The antiviral mechanism of CCCH-zinc finger antiviral protein against Avian leukosis virus subgroup J[D].Tai'an:Shandong Agricultural University,2020.(in Chinese)
[29] KAPCZYNSKI D R,JIANG H J,KOGUT M H.Characterization of cytokine expression induced by Avian influenza virus infection with Real-time RT-PCR[J].Methods in Molecular Biology,2014,1161:217-233.
[30] CHEN S,LUO G,YANG Z,et al.Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways[J].Veterinary Research,2016,47(1):74.
[31] WANG S,XIE L,XIE Z,et al.Dynamic changes in the expression of interferon-stimulated genes in joints of SPF chickens infected with Avian reovirus[J].Frontiers in Veterinary Science,2021,8:618124.
[32] 谢智文,曹艳杰,何怡宁,等.干扰素刺激基因在鸡传染性支气管炎病毒感染雏鸡气管黏膜中表达的动态变化[J].动物医学进展,2016,37(3):19-24. XIE Z W,CAO Y J,HE Y N,et al.Dynamic changes in expression of interferon stimulated genes in trachea mucosa of chickens infected with IBV[J].Progress in Veterinary Medicine,2016,37(3):19-24.(in Chinese)
[33] SMITH S E,GIBSON M S,WASH R S,et al.Chicken interferon-inducible transmembrane protein 3 restricts Influenza viruses and Lyssaviruses in vitro[J].Journal of Virology,2013,87(23):12957-12966.
[34] XIE L,XIE Z,WANG S,et al.Altered gene expression profiles of the MDA5 signaling pathway in peripheral blood lymphocytes of chickens infected with Avian reovirus[J].Archives of Virology,2019,164(10):2451-2458.
[35] DAVIET S,VAN BORM S,HABYARIMANA A,et al.Induction of Mx and PKR failed to protect chickens from H5N1 infection[J].Viral Immunology,2009,22(6):467-472.
[36] 栾勇娇,谢芝勋,王盛,等.血清4型禽腺病毒感染SPF鸡体内各组织中病毒的动态分布及排毒规律[J].中国兽医学报,2021,41(3):463-468. LUAN Y J,XIE Z X,WANG S,et al.Dynamic distribution of infectious Fowl adenovirus serotype 4 in different tissues and the virus shedding pattern of SPF chickens[J].Chinese Journal of Veterinary Science,2021,41(3):463-468.(in Chinese)
[37] MAIER B D,AGUILERA L U,SAHLE S,et al.Stochastic dynamics of type-Ⅰ interferon responses[J].PLoS Computational Biology,2022,18(10):e1010623.
[38] 王祖贵.肉鸡感染FAdV-4后肝脏组织损伤及炎症相关基因表达研究[D].杨凌:西北农林科技大学,2017. WANG Z G.Tissue damage and inflammation-related genes expression in the liver of broilers infected with FAdV-4[D].Yangling:Northwest A&F University,2017.(in Chinese)
[39] 熊修琦.血清4型禽腺病毒的分离鉴定及鸡Viperin蛋白对其复制的影响[D].合肥:安徽农业大学,2021. XIONG X Q.Isolation and identification of Fowl adanovirus type 4 and the effect of chicken Viperin protein on its replication[D].Hefei:Anhui Agricultural University,2021.(in Chinese)

Dynamic Changes in Expression of Interferon-stimulated Genes at the Transcriptional Level After Infection with Fowl Adenovirus Serotype-4 in LMH cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Kuan, YANG Bowen, WANG Juyu, DENG Jianming, XU Hui, YANG Yang, CHEN Hongjian, DAI Feiyan, GU Xiaolong, QU Weijie, ZHANG Limei. Regulation of Epithelial Mesenchymal Transition in Mammary Epithelial Cells of Dairy Cow by RUNX1 [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 500-512.
[2]	CHEN Hongling, ZHAO Yi, CHEN Jiaji, WEI Qiuxu, LI Ximeng, FENG Guoyue, HU Kunxiang, HU Tingjun. Transcriptomic Analysis of 3D4/2 Cells Infected with Porcine Circovirus Type Ⅱ [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 42-51.
[3]	CHEN Lin, WANG Jiaxiang, WU Yan, PI Jinsong, ZHANG Ying, LI Chengfeng. Functional Analysis, Core Promoter Screening and Transcription Factors Prediction of PERP1 Gene in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3449-3458.
[4]	DENG Zhichao, ZHANG Cheng, LUO Pei, ZHAN Xiaozhi, JIANG Danli, YANG Youtian, LIU Wenjun. Cloning and Tissue Expression Specificity Analysis of Interferon Regulatory Factor 7 Gene in Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2606-2616.
[5]	MA Yong, CHEN Zongchang, LI Xupeng, LI Jingsheng, BAI Yanbin, LIU Zhanxin, WEI Yali, GUO Dashan, SHI Bingang, ZHAO Zhidong, LUO Yuzhu, HU Jiang, WANG Jiqing, LIU Xiu, LI Shaobin. Cloning and Transcription Factor Prediction of PLAG1 Gene in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2661-2669.
[6]	SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of MyoG Gene Promoter Region Sequence in Guizhou White Goat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 893-903.
[7]	QIN Rongfen, JIA Qiong, YU Leitao, LI Jiamin, CHI Zhirui, FAN Ruiwen. Preparation and Functional Identification of STAT3 Nanobody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1118-1128.
[8]	SONG Shiying, GUO Weilu, XIA Xuefeng, ZHANG Xue, BI Zhenwei, ZHANG Xuehan, FAN Baochao, DONG Hailong, LI Bin. Expression and Purification of Porcine IFN-δ5 and Its Effect Analysis Against PEDV Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3171-3179.
[9]	DU Xinze, MA Xinhao, ZHANG Dianqi, DU Jiawei, MA Jing, XIE Kuncheng, HE Jie, ZAN Linsen. Target Gene Prediction and Bioinformatics Analysis of bta-miR-34b/c and bta-miR-449a/b/c [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2451-2461.
[10]	ZHAI Zhe, CHEN Si, WU Yanru, WANG Xuemei, LI Chongrui, LIU Zhiyong, CHEN Qiaoling, WANG Fengyang, DU Li, LI Chang, JIN Ningyi. Analysis of Promoter Activity and Screening of Transcription Regulatory Elements of CCL19 Gene in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1213-1222.
[11]	CHEN Zhe, YANG Pengxia, LEI Mingming, CHEN Jiabin, YAN Leyan. Cloning, Promoter Analysis and Transcriptional Regulation of p21 Gene in Goose [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 405-415.
[12]	PANG Feng, LONG Qinqin, LIANG Shaobo, CHENG Hongsong, CHENG Zhentao. Bioinformatics Analysis,Eukaryotic Expression and Subcellular Localization of Orf Virus ORFV114 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4150-4158.
[13]	LI Lingyu, ZHENG Haiying, CHEN Mingtang, TANG Liping, SHANG Jianghua, YANG Chunyan. Effects of Oocyte Origin on the Developmental Potential of Buffalo Somatic Cell Nuclear Transfer Embryos [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 241-247.
[14]	LYU Qiao, ZOU Chaoxia, WU Yongmei, ZHANG Ruimen, ZHENG Zihua, SHI Deshun, WEI Yingming, DENG Yanfei. Study on Regulation of STAT3 Gene on Myogenic Differentiation of Muscle Stem Cells in Guangxi Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2771-2777.
[15]	YU Jie, HU Xiuzhong, XIANG Min, WANG Dingfa, TAO Bifei, XIA Yu, TAO Hu, XIONG Qi, CHENG Lei. Effects of Arginine on Expression of Genes in Bovine Endometrial Epithelial Cells Treated with Interferon-tau [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2495-2503.