鸡传染性法氏囊高免卵黄IgY抗体不同提取方法的比较

Abstract

Abstract: In order to improve prerention and control of infectious bursal IgY effect of infectious bursal,comparison of different purification method on the high IBD yolk antibody titer of effection. Preparation of hyper-immune eggs and determination of the titer,in the lab, four methods were used for extraction high-yolk antibodies and determination of the titer.They were direct centrifugation, acidfied water extraction, chloroform extraction method and polyethylene glycol method. The results showed that self-prepared yolk antibody titer was 1:64, direct centrifugation antibody titer was 1:128, acidfied water extraction antibody titer was 1:16, chloroform extraction method antibody titer was 1:256, polyethylene glycol method antibody titer was 1:32.The results showed that after using titer chloroform extraction method,the highest titer detected, chloroform extraction method was a common method of clinical extracted antibody.

Key words: bursa; hyper-immune yolk; extraction of antibody; titer detection

CLC Number:

S852.4⁺3

GUO Su-qin, WU Feng-sun. Comparison of Different Extraction Methods of Chicken Infectious Bursal Disease the Yolk Antibody IgY[J]. , 2012, 39(7): 238-240.

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Comparison of Different Extraction Methods of Chicken Infectious Bursal Disease the Yolk Antibody IgY

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