传染性法氏囊病病毒VP4酵母双杂交诱饵载体的构建及互作宿主细胞蛋白的筛选

doi:10.16431/j.cnki.1671-7236.2015.02.012

Abstract

Abstract: Viral protein 4 (VP4) is an important non-structural protein with proteolytic enzyme activity encoded by infectious bursal disease virus (IBDV) genome that catalyzes the hydrolysis of polyprotein pVP2-VP4-VP3 to form viral proteins pVP2,VP4,VP3,which plays an important role in the process of viral proteins maturity.To study the interaction between VP4 and the host cell,VP4 full-length cDNA sequence was amplified and inserted into pGBKT7 vector to construct a bait vector (pGBGtVP4).The result of autoactivation assay showed that pGBGtVP4 vector had no autoactivation and virulence in Y2H gold yeast cell.After screening,sequencing and confirming interaction,22 kinds of potential interacted proteins were obtained from the expression library of chicken embryo fibroblasts (CEF) using the matchmaker gold yeast two-hybrid system.This study laid the foundation for further researches on the effect and mechanism of the interaction of IBDV VP4 and its host cell.

Key words: infectious bursal disease virus (IBDV); VP4; yeast two-hybrid; bait vector; interacted protein

CLC Number:

Q785

WANG Nian, CHEN Yu-ming, ZHANG Li-zhou, GAO Li, LU Zhen, GAO Yu-long, WANG Yong-qiang, GAO Hong-lei, LIU Chang-jun, CUI Hong-yu, WANG Xiao-mei, QI Xiao-le. Construction of Bait Vector of Infectious Bursal Disease Virus VP4 and Screening of the Interacted Proteins of Host Cell Using Yeast Two-hybrid System[J]. , 2015, 42(2): 317-323.

References

[1] Muller H,Islam M R,Raue R.Research on infectious bursal disease-The past,the present and the future[J].Vet Microbiol,2003,97(1):153-165.
[2] Azad A A,Jagadish M N,Brown M A,et al.Deletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus[J].Virology,1987,161(1):145-152.
[3] Granzow H,Birghan C,Mettenleiter T C,et al.A second form of infectious bursal disease virus-associated tubule contains VP4[J].J Virol,1997,71(11):8879-8885.
[4] Li Z,Wang Y,Li X,et al.Critical roles of glucocorticoid-induced leucine zipper in infectious bursal disease virus (IBDV)-induced suppression of type Ⅰ interferon expression and enhancement of IBDV growth in host cells via interaction with VP4[J]. J Virol,2013,87(2):21-31.
[5] 祁小乐,陈玉明,张礼洲,等.鸡胚成纤维细胞cDNA 酵母表达文库的构建与鉴定[J].中国家禽,2013,35(8):14-16.
[6] Fromont-Racine M,Rain J C,Legrain P.Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens[J]. Nat Genet,1997,16(1):277-282.
[7] 陈玉明,张礼洲,任宪刚,等.不同毒力传染性法氏囊病病毒衣壳蛋白酵母双杂交诱饵载体的构建及鉴定[J].中国畜牧兽医,2014,41(1):30-33.
[8] Gietz R D,Woods R A.Screening for protein-protein interactionsin the yeast two-hybrid system[J]. Methods Mol Biol,2002,185:471-486.
[9] White M A.The yeast two-hybrid system: Forward and reverse[J]. Proc Natl Acad Sci USA,1996,93(19):10001-10003.
[10] Browning C L,Culberson D E,Aragon I V,et al.The developmentally regulated expression of serum response factor plays a key role in the control of smooth muscle-specific genes[J].Developmental Biology,1998,194(1):18-37.
[11] Birghan C,Mundt E,Gorbalenya A E.A non-canonical lon proteinase lacking the ATPase domain employs the Ser-Lys catalytic dyad to exercise broad control over the life cycle of a double-stranded RNA virus[J]. Embo J,2000,19(1):114-123.
[12] Lejal N,Da Costa B,Huet J C,et al.Role of Ser-652 and Lys-692 in the protease activity of infectious bursal disease virus VP4 and identification of its substrate cleavage sites[J].J Gen Virol,2000,81(4):983-992.
[13] Sanchez A B,Rodriguez J F.Proteolytic processing in infectious bursal disease virus: Identification of the polyprotein cleavage sites by site-directed mutagenesis[J].Virology,1999,262(1):190-199.
[14] Chang G R,Wang M Y,Liao J H,et al.Endopeptidase activity characterization of E.coli-derived infectious bursal disease virus protein 4 tubules using flow cytometry[J].Arch Virol,1998,143(12):2327-2341.
[15] Manel N,Hogstad B,Wang Y,et al.A cryptic sensor for HIV-1 activates antiviral innate immunity in dendritic cells[J]. Nature,2010,467(7312):214-217.
[16] Takemura T,Kawamata M,Urabe M,et al.Cyclophilin A dependent restriction to capsid N121K mutant human immunodeficiency virus type Ⅰ in a broad range of cell lines[J].J Virol,2013,87(7):4086-4090.
[17] Liu Z,Yang F,Robotham J M,et al.Critical role of cyclophilin A and its prolyl-peptidyl isomerase activity in the structure and function of the hepatitis C virus replication complex[J].J Virol,2009,83(13):6554-6665.
[18] Liu X,Sun L,Yu M.Cyclophilin A interacts with influenza A virus M1 protein and impairs the early stage of the viral replication[J].Cellular Microbiology,2009,11(5):730-741.
[19] Liu X L,Zhao Z D,Liu W J.Insights into the roles of cyclophilin A during influenza virus infection[J].Viruses,2013,5(1):182-191.
[20] Stephen D,Emily L,Hengli T,et al.Cyclophilins as modulators of viral replication[J].Viruses,2013,5(7):1684-1701.
[21] Luban J,Goff S P.The yeast two-hybrid system for studying: Protein-protein interaction[J].Curr Opin Biotech,1995,6(1):59-64.

Construction of Bait Vector of Infectious Bursal Disease Virus VP4 and Screening of the Interacted Proteins of Host Cell Using Yeast Two-hybrid System

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DAN Yixin, XIANG Hua, ZHANG Huanrong, YANG Lu, REN Yupeng, XU Songwei, HE Honghong, ZHU Jiangjiang. Screening of Host Proteins Interacting with ORFV129 Protein and Cloning and Analysis of C1QBP Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 1-14.
[2]	QIU Runhui, GUAN Feihu, TAO Tingting, LI Jia, ZHAO Tianyi, DENG Xingmei, SHI Chao, SUN Zhihua, ZHANG Hui. Subcellular Localization and Screening of Host Interacting Proteins of Brucella Secreted Protein BPE005 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1438-1448.
[3]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[4]	CUI Shuai, WANG Yang, GUO Xiaoyu, CHEN Shiyu, JIANG Yajun, PANG Zhongbao, HUANG Jianxin, MENG Fanfeng, JIANG Yitong, ZHU Hongfei, JIA Hong. Screening and Identification of the Host Proteins Interacting with African Swine Fever Virus E120R Protein Using Yeast Two-hybrid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4139-4149.
[5]	MENG Weijin, FAN Kuohai, CHANG Xiongxiong, YIN Wei, LI Hongquan, WANG Zhirui. Eukaryotic Expression and Immunogenicity Detection of Fusion Protein of Chicken Infectious Bursal Disease Virus VP2 and Truncated Diphtheria Toxin DT390 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4652-4660.
[6]	WANG Yanwei, ZHANG Suling, WU Peng, GENG Xiaolin, HUANG Yuxin, LI Penghao, WANG Mengyue, LI Xiangdong, PANG Wenqiang, TIAN Kegong. Identification of Subviral Particles Efficiently Self-assembled by Infectious Bursal Disease Virus rVP2 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1677-1684.
[7]	GAO Xiang, LI Hui, ZHANG Li-zhou, LU Zhen, WANG Yong-qiang, GAO Li, WU Tian-tian, GAO Yu-long, LIU Chang-jun, WANG Xiao-mei, QI Xiao-le. Soluble Expression, Purification and Activity Analysis of Capsid Protein of Very Virulent Infectious Bursal Disease Virus [J]. , 2017, 44(7): 2096-2102.
[8]	LU Heng, XIE Zhi-xun, XIE Li-ji, HUANG Li, HUANG Jiao-ling, WANG Sheng, ZHANG Yan-fang, ZENG Ting-ting, FAN Qing, LUO Si-si, XIE Zhi-qin, DENG Xian-wen. Construction and Identification of Bait Vector of Avian Reovirus σA Gene in Yeast Two-hybrid System [J]. , 2017, 44(3): 847-853.
[9]	WANG Jie-qiong, ZHAO Yu-jie, ZHOU Yun-fei, HUANG Zong-mei, CHEN Pan-pan, ZHOU Wei-fan, LIU Lin, LI Xin-sheng. Prokaryotic Expression and Bioinformatics Analysis of VP2 Gene of Infectious Bursal Disease Virus C4 Strain [J]. , 2017, 44(12): 3585-3591.
[10]	CHI Yan-bin，SHI Da，ZHU Qing-he，CHEN Jian-fei，SHI Hong-yan，ZHANG Xin，LI Chang-long，HAN Xiao，LIU Ning，ZHAO Xin，FENG Li. Construction and Characterization of the Yeast Two-hybrid cDNA Library of Vero E6 Cells [J]. , 2014, 41(3): 124-127.
[11]	XU Tian, SHI Da, CHEN Jian-fei, GU Feng-li, SHI Hong-yan, ZHANG Xin, FENG Li. Construction and Identification of Yeast Two-hybrid cDNA Library of Porcine Intestinal Epithelial Cells [J]. , 2014, 41(10): 33-37.
[12]	CHEN Yu-ming, ZHANG Li-zhou, REN Xian-gang, GAO Li, QIN Li-ting, GAO Yu-long, WANG Yong-qiang, GAO Hong-lei, WANG Xiao-mei, QI Xiao-le. Construction and Identification of Yeast Two-hybrid Bait Vectors of Capsid Protein of IBDV Different Virulence Strains [J]. , 2014, 41(1): 30-34.
[13]	ZHENG Li-shuang, LI Xiang-nan, SUN Cheng-tao, LIU Hong-yu, LIU Xun, HE Ming, LV Wen-fa. Research Progress on Yeast Two-hybrid Technology and Application [J]. , 2013, 40(9): 105-108.
[14]	WU Yi-chun. Study and Application on an Immunochromatographic Method for Detection the VP4 Protein Antibody of Infectious Bursal Disease Virus [J]. , 2013, 40(6): 65-69.
[15]	CUI Peng-peng, GAO Hong-lei, LI Kai, GAO Li, GAO Yu-long, QI Xiao-le, WANG Xiao-mei. Construction and Characterization of Yeast Two-hybrid cDNA Expression Library of MDCC-MSB1 [J]. , 2013, (5): 22-25.