鸡传染性法氏囊病病毒BJQ902株在DF-1细胞系上增殖工艺研究

doi:10.16431/j.cnki.1671-7236.2016.10.039

Abstract

Abstract:

In order to produce high titers of infectious bursal disease virus(IBDV) antigen,the proliferation technology of chicken IBDV BJQ902 strain in DF-1 cell line was studied by the selection and optimization of the following five culture conditions,including the amount of inoculated virus,harvest time,inoculation time,culture temperature and serum concentration in maintenance media.The results showed that the optimal proliferation conditions of IBDV BJQ902 strain in DF-1 cell line were obtained as follow:The culture temperature was 37℃,the inoculum concentration was between 0.01% and 0.1% (V/V),the inoculation time was between 48 and 72 h after cell passage,the serum concentration in maintenance media was between 1% and 2%,the harvest time was between 60 and 72 h after inoculation.Under the optimal conditions,the virus titers were between 10^8.3 and 10^8.7 TCID₅₀/0.1 mL.

Key words: infectious bursal disease virus; BJQ902 strain; DF-1 cell line; virus titer

CLC Number:

S852.65⁺9.4

ZHANG Zhen-hua, LI Lin, JING Xiao-dong, ZHANG Jian-wei, SHEN Jia, SHI Ai-hua, ZHENG Xiao-lan, HUANG Feng-jun, JIANG Bei-yu. Study on Proliferation Technology of Chicken Infectious Bursal Disease Virus BJQ902 Strain in DF-1 Cell Line[J]. , 2016, 43(10): 2775-2779.

References

[1] Saif B W主编.高福,苏敬良,索勋主译.禽病学[M].第12版.北京:中国农业出版社,2012.
[2] 殷震,刘景华.动物病毒学[M].第2版.北京:科学出版社,1997.
[3] 于建立,王雷,魏波,等.鸡传染性法氏囊病疫苗应用现状与研究进展[J].安徽农业科学,2016,4(1):91-92.
[4] 梅永杰,王永山,欧阳伟,等.华东地区传染性法氏囊病病毒流行株的分离及其生物学特性分析[J].中国兽医科学,2016,46(5):550-557.
[5] 王寿山,毛雅元,张立霞,等.4株鸡传染性法氏囊病病毒VP2基因序列分析[J].中国畜牧兽医,2013,40(12):48-51.
[6] 章振华,李林,景小冬,等.鸡传染性法氏囊病免疫复合物疫苗对1日龄低母源抗体水平雏鸡免疫效果的研究[J].中国畜牧兽医,2015,42(9):2493-2498.
[7] 章振华,李林,景小冬,等.鸡传染性法氏囊病免疫复合物疫苗对1日龄中等母源抗体水平雏鸡免疫效果的研究[J].中国畜牧兽医,2015,42(12):3338-3343.
[8] 中国兽药典委员会编.中华人民共合作兽药典(三部)[M].北京:中国农业出版社,2011.
[9] 万桂青,丁献红,贾灵霞.采用继代鸡胚成纤维细胞生产鸡传染性法氏囊细胞苗的试验[J].中国兽药杂志,2005,39(8):51-52.
[10] 顾铭,聂峰光,戚艺华,等.传染性法氏囊病病毒弱毒株对Vero细胞的适应性研究[J].生命科学研究,2000,4(3):197-201.
[11] 石岗,王宏俊,孙惠玲,等.传染性法氏囊病病毒Vero细胞培养条件优化的研究[J].中国兽医杂志,2000,26(4):5-7.
[12] 王永生,周欣,杨霞.鸡传染性法氏囊病毒在DF-1细胞系上繁殖特性研究[J].河南农业大学学报,2011,45(6):662-667.
[13] 高诗敏,马海利,邓瑞林,等.鸭源、鸡源NDV对DF-1细胞的致病性特征[J].农业科技与信息,2016,6:128-129.
[14] 刘玉鹏,赖汉漳,李延鹏,等.马立克氏疫苗病毒在鸡传代细胞上的培养工艺[J].中国兽医学报,2014,34(8):1244-1247.
[15] 史爱华,姜北宇,沈佳,等.3株H9亚型禽流感病毒在3种传代细胞系上增殖效果研究[J].黑龙江畜牧兽医,2013,11:111-113.
[16] Wang Y,Qi X,Gao H,et al.Comparative study of the replication of infectious bursal disease virus in DF-1 cell line and chicken embryo fibroblasts evaluated by a new Real-time RT-PCR[J].J Virol Methods,2009,157(2):205-210.
[17] Rekha K,Sivasubramanian C,Chung I M,et al.Growth and replication of infectious bursal disease virus in the DF-1 cell line and chicken embryo fibroblasts[J].Biomed Res Int,2014,2014:494835.
[18] 张立,张元,严春,等.传染性法氏囊病病毒在鸡胚细胞上的优化繁殖[J].华东理工大学学报,1996,42(60):707-711.
[19] 戚凤春,汪春义,张雪梅,等.两种细胞培养流感病毒的滴度比较[J].中国生物制品学杂志,2006,19(3):291-292.
[20] 陈昌海,程雷,杨瑛,等.鸡传染性法氏囊炎二价细胞苗最佳培养条件的选择[J].中国兽药杂志,1995,3:12-15.

Study on Proliferation Technology of Chicken Infectious Bursal Disease Virus BJQ902 Strain in DF-1 Cell Line

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[2]	MENG Weijin, FAN Kuohai, CHANG Xiongxiong, YIN Wei, LI Hongquan, WANG Zhirui. Eukaryotic Expression and Immunogenicity Detection of Fusion Protein of Chicken Infectious Bursal Disease Virus VP2 and Truncated Diphtheria Toxin DT390 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4652-4660.
[3]	WANG Yanwei, ZHANG Suling, WU Peng, GENG Xiaolin, HUANG Yuxin, LI Penghao, WANG Mengyue, LI Xiangdong, PANG Wenqiang, TIAN Kegong. Identification of Subviral Particles Efficiently Self-assembled by Infectious Bursal Disease Virus rVP2 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1677-1684.
[4]	HAN Wei, ZHOU Jianmin, HAO Linyu, PAN Wen, PAN Jingxue. Study on the Reproductive Characteristics of Swine Japanese Encephalitis Virus in Vero Cells and the Immunogenicity of Its Inactivated Vaccine [J]. , 2018, 45(12): 3572-3578.
[5]	GAO Xiang, LI Hui, ZHANG Li-zhou, LU Zhen, WANG Yong-qiang, GAO Li, WU Tian-tian, GAO Yu-long, LIU Chang-jun, WANG Xiao-mei, QI Xiao-le. Soluble Expression, Purification and Activity Analysis of Capsid Protein of Very Virulent Infectious Bursal Disease Virus [J]. , 2017, 44(7): 2096-2102.
[6]	LI Ji-dong, CAI Xue-peng. Effect on Proliferation of Goat Pox Virus of TK Gene Deficiency [J]. , 2017, 44(2): 319-326.
[7]	WANG Jie-qiong, ZHAO Yu-jie, ZHOU Yun-fei, HUANG Zong-mei, CHEN Pan-pan, ZHOU Wei-fan, LIU Lin, LI Xin-sheng. Prokaryotic Expression and Bioinformatics Analysis of VP2 Gene of Infectious Bursal Disease Virus C4 Strain [J]. , 2017, 44(12): 3585-3591.
[8]	WANG Nian, CHEN Yu-ming, ZHANG Li-zhou, GAO Li, LU Zhen, GAO Yu-long, WANG Yong-qiang, GAO Hong-lei, LIU Chang-jun, CUI Hong-yu, WANG Xiao-mei, QI Xiao-le. Construction of Bait Vector of Infectious Bursal Disease Virus VP4 and Screening of the Interacted Proteins of Host Cell Using Yeast Two-hybrid System [J]. , 2015, 42(2): 317-323.
[9]	CHEN Yu-ming, ZHANG Li-zhou, REN Xian-gang, GAO Li, QIN Li-ting, GAO Yu-long, WANG Yong-qiang, GAO Hong-lei, WANG Xiao-mei, QI Xiao-le. Construction and Identification of Yeast Two-hybrid Bait Vectors of Capsid Protein of IBDV Different Virulence Strains [J]. , 2014, 41(1): 30-34.
[10]	YANG Feng, LI Xian-wei, SUN Min-hua, YAN Fu-qiang, WEI Qing-lan, XIANG Bin, REN Tao. Expression of VP2 Gene of Infectious Bursal Disease Virus in Insect Cells [J]. , 2013, 40(9): 65-69.
[11]	WU Yi-chun. Study and Application on an Immunochromatographic Method for Detection the VP4 Protein Antibody of Infectious Bursal Disease Virus [J]. , 2013, 40(6): 65-69.
[12]	WANG Shou-shan, MAO Ya-yuan, ZHANG Li-xia, KANG Ya-nan, SUN Mei-yu, CHEN Bing, LV Mao-jie, HE Zhao-qing, YANG Bao-shou. Sequence Analysis of VP2 Gene of Four Infectious Bursal Disease Virus Strains [J]. , 2013, 40(12): 48-51.
[13]	YU Wen-lan, ZHANG Xiao-zhan, HUANG Hong-liang, LIU Bi-tao, REN Chang-bao, TANG Zhao-xin. Study on Proliferation Characteristics and Pathogenicity of Pseudorabies Virus Ra Strain in Different Cells [J]. , 2013, 40(11): 164-168.
[14]	SONG Tie-bin;CHEN Guang-dong. Preliminary Establishment of Indirect ELISA Based on the VP3 Structural Protein of IBDV [J]. , 2011, 38(1): 116-118.
[15]	CHENG Cong;ZHANG Min-hong;ZHENG Shan-shan;FENG Jing-hai;ZHAO Chun-fu. Effects of Sulfated Glucomannan on the Antiviral Activity of Broiler Infected with IBDV [J]. , 2010, 37(8): 157-159.