Abstract: African swine fever (ASF) is an acute,febrile and highly contagious infectious disease caused by African swine fever virus (ASFV).Both morbidity and mortality rate of acute cases are almost 100%.ASF is recognized as a statutory report animal disease by the World Organization for Animal Health (OIE) and listed as class Ⅰ animal disease in China.At present,the situation of ASF epidemic prevention and control in China is very serious,and there is no effective vaccine and preventive and therapeutic agents for ASF.Therefore,strict sanitation and removal of infected animals through monitoring pathogen and antibody are important measures for control of ASF.In this paper,we summarized the recent progress in development of detection and serological monitoring methods for ASF built on its specific diagnostic marker.In etiological diagnosis,isothermal amplification techniques developed on the basis of PCR,such as recombinase polymerase amplification,crossing-priming amplification,loop-mediated isothermal amplification and dropletdigital PCR,overcome the defects of conventional PCR in different aspects.Real-time quantitative PCR provides fast,accurate,objective and quantitative results.The new CRISPR/Cas12a technology does not require an expensive instrument and offers a new,more portable,simple,sensitive and specific alternative to ASFV detection.On serological diagnosis,based on enzyme-linked immunosorbent assay (ELISA),immunochromatography and indirect immunofluorescence assay have also improved sensitivity and specificity.

Key words: African swine fever (ASF); diagnostic method; antigen; serology