胚胎移植前供体牛与受体牛血浆外泌体miRNA差异表达分析

doi:10.16431/j.cnki.1671-7236.2022.02.026

Abstract

Abstract: 【Objective】 This study was to explore the difference in the expression of plasma exosome miRNA between donor and recipient cattle before embryo transfer, and to detemine the role of plasma exosome miRNA in bovine pregnancy.【Method】 Xianan cattle aged 3-6 years and weighing 480-600 kg were used as the research object, 10 donor cattle were selected for simultaneous estrus, superovulation and artificial insemination, and 23 recipient cattle were treated for simultaneous estrus. On the 7th day after artificial insemination, the donor bovine uterus was washed to obtain blastocysts, 3 donor cattle with similar number of blastocysts and three recipient cattle with similar body weight and age to the donor cattle were selected to collect blood from jugular vein, and plasma exosomes were collected.After extracting miRNA from plasma exosomes, the expression level was analyzed.Target genes of differentially expressed miRNA were predicted.While the P value was calculated with DESeq algorithm in R package, the miRNA with P < 0.05 were screened, and the differentially expressed miRNA were analyzed by target gene prediction, GO function enrichment analysis and KEGG signaling pathway analysis.【Result】 The vesicles particle size of the 6 samples was about 135 nm, which was consistent with the diameter characteristics of exosomes.Nine miRNAs were significantly up regulated in recipient cattle compared with donor cattle (P < 0.05), and 13 miRNAs were significantly down regulated (P < 0.05).Among the 22 differentially expressed miRNAs, 15 miRNAs predicted 2 990 target genes without duplication.The results of GO function enrichment analysis and KEGG signal pathway analysis indicated that these target genes were mainly concentrated in the functional pathways related to biological adhesion, localization and cell junction, and significant accumulation of signal pathway was related to focal adhesion and adherens junction, suggested that plasma exosomal miRNA might be involved in the regulation of embryo implantation.【Conclusion】 These results could provide reference for screening and exploring the plasma exosomal miRNA affecting embryo implantation, and provide a basis for further elucidation of the role of plasma exosomal miRNA in the regulation of early pregnancy in cattle.

Key words: cattle; exosome; miRNA; differential expression; pregnancy

CLC Number:

Q251

ZHAI Yaying, SHI Qiaoting, CHU Qiuxia, ZHU Xiaoting, HUA Liushuai, ZHANG Zijing, CHEN Fuying, QI Xinglei, WANG Eryao, LYU Shijie. Differential Expression Analysis of Plasma Exosomes microRNA Between Donor and Recipient Cattle Before Embryo Transfer[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 641-649.

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Differential Expression Analysis of Plasma Exosomes microRNA Between Donor and Recipient Cattle Before Embryo Transfer

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