TaqMan探针荧光RT-PCR检测狂犬病病毒

Abstract

Abstract: To establish the method of TaqMan probe real-time RT-PCR for detection of rabies virus, a pair of primers and probe were designed based on the nucleoprotein sequences of rabies virus that published in GenBank. RNA was extracted from rabies virus vaccine, and then amplified by RT-PCR, cloned the target fragment and took the recombination plasmid as standard positive control. The results showed that the method can distinguished rabies virus from canine distemper virus, canine parvovirus, canine adenoviruses, canine coronavirus and canine para influenza virus, the sensitivity could attained 10 copies/μL and the stability test was good.

Key words: rabies virus; TaqMan probe; real-time RT-PCR

CLC Number:

S854.4

WANG Zhen-quan, LUO Bao-zheng, BO Qing-ru, ZHOU Chang-fang, BAI Ge, XU Yuan-jing, WANG Chong, WU Dian-jun. Detection of Rabies Virus by TaqMan Probe Real-time RT-PCR[J]. , 2012, 39(6): 79-82.

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Detection of Rabies Virus by TaqMan Probe Real-time RT-PCR

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