犬瘟热病毒贵州株(CDV-GZ1)F基因的克隆及序列分析

Abstract

Abstract: Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank, and the F gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) from CDV-GZ1 strain. The amplified fragment was cloned and analyzed. The results showed that the length of CDV-GZ1 F gene was 1989 bp. It contained five potential N-glycosylation locations based on the derived amino acid sequences. The homology of coding amino acid between this strain and American 00-2601 and domestic HL strain was 91.6% and 91.1% respectively, it showed that they had common ancestor.

Key words: canine distemper virus; CDV-GZ1; fusion protein; cloning; sequence analysis

CLC Number:

ZENG Zhi-yong, ZHOU Li, LIU Zhi-jie. Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Guizhou[J]. , 2012, 39(6): 53-56.

References

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Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Guizhou

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