鸽源阿哥纳沙门菌的分离鉴定和生物学特性研究

doi:10.16431/j.cnki.1671-7236.2020.06.035

Abstract

Abstract: In order to understand the cause of the disease in a pigeon farm in Yunnan,a treatment plan was developed.The liver tissues of sick pigeons were collected aseptically and streaked on MacConkey medium for bacterial isolation and culture,biochemical identification,serotype identification,16S rDNA homology analysis,animal regression experiments,drug sensitivity tests and virulence analysis.The results showed that round,smooth,colorless and translucent colonies were observed on the MacConkey medium,which were initially identified as Gram-negative bacteria.The strain was identified as Salmonella Agona by Salmonella serum agglutination test and 16S rDNA homology analysis.Its antigenic structural formula was O antigen 1(+),4(+),12(+),H antigen fg(+),s(+),which was named SSYN036 strain.The growth curve of bacteria showed that the strain grow faster.Animal experiments showed the strain was highly pathogenic to the test mice,the inoculated with 5×10⁸,5×10⁷,5×10⁶ and 5×10⁵ CFU/mL of mice,within 7 days,which mortality were 100%,100%,60% and 30%,respectively.The pathological sections showed more obvious focal necrotic inflammation and inflammatory cell infiltration.The drug sensitivity test showed that the strain was resistant to penicillin,oxacillin,streptomycin,doxycycline and tetracycline,moderately sensitive to rifampicin,and sensitive to amoxicillin,ampicillin,gentamicin,tobramycin,ceftazidime,etc.11 antibacterial drugs.Virulence gene test showed that the strain contains spvB,spiA,pagC,msgA,invA,sipB,prgH,spaN,tolC,iroN,sitC,lpfC,sifA,sopB and pefA genes.This study provided new data information of the pathogenicity of Salmonella and the biological characteristics of strains,and its public health significance was worthy of attention.

Key words: pigeon; Salmonella Agona; isolation and identification; biological characteristics; drug susceptibility test

CLC Number:

S852.61

LI Tingcui, LI Ke, YAN Hongya, ZHAO Rong, TANG Yaqi, CHANG Zhishun, ZOU Fengcai, XIN Aiguo. Isolation,Identification and Biological Characteristics of a Pathogon of Salmonella Agona Originated from Pigeon[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1952-1960.

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Isolation,Identification and Biological Characteristics of a Pathogon of Salmonella Agona Originated from Pigeon

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