浙江省猪繁殖与呼吸综合征病毒ORF5基因的遗传变异分析

doi:10.16431/j.cnki.1671-7236.2019.12.031

Abstract

Abstract: To assess the genetic diversity of ORF5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in Zhejiang province from 2014 to 2016,ORF5 gene was amplified and sequenced in the PRRSV positive samples which were collected from different places of Zhejiang province.The homologous analysis and genetic analysis were conducted by DNAStar and Mega 5.1 softwares.Homologous analysis showed that ORF5 gene of 54 PRRSV strains shared 83.1% to 100% nucleotide homology and 80.6% to 100% amino acid homology.ORF5 gene of 54 PRRSV strains shared 83.8% to 90.4% nucleotide homology and 81.6% to 90.0% amino acid homology with classical strain VR2332.ORF5 gene of 54 PRRSV strains shared 83.5% to 99.5% nucleotide homology and 83.1% to 99.0% amino acid homology with virulent strain JXA1.The phylogenetic tree based on sequences of ORF5 gene suggested the 54 strains could be classified in subgenotypes Ⅰ,Ⅲ and Ⅳ,among them,subgenotypes Ⅲ and Ⅳ were new strains in Zhejiang province in recent years.Compared with antigen epitopes,54 PRRSV strains had higher variation in the signal epitope and non-neutralizing antigen epitopes,and had little variation in neutralizing epitope.The results indicated that four subtypes of PRRSV existed in Zhejiang province,but mainly prevailing the subgenotype Ⅰ PRRSV strain.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); ORF5 gene; genetic variation

CLC Number:

S852.65

WANG Yating, CHAI Juan, ZHANG Hongli, LIU Xia, YU Yicong, NI Baifeng, ZHAO Lingyan, XU Hui, XIE Ronghui. Genetic Variation Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in Zhejiang Province[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3715-3724.

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Genetic Variation Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in Zhejiang Province

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