Abstract: From 2006 to 2008,116 hepatitis B positive serums were detected in 610 dairy cow serums in Guizhou province, the mean positive ratio of HBsAg was 19%(116/610). 29 infectious bovine rhinotracheitis positive serums were detected by neutralization test in 534 dairy cow serums, the positive ratio was 5.4%(29/534). The antibody of dairy cow contagious pleuropneumonia were negative in 534 dairy cow serums by CFT. HBV particles were observed by electron microscopic observation. The results indicated that dairy cow type B hepatitis and infectious rhinotracheitis existed probably in Guizhou province. The breeding cow industry should pay close attention to dairy cow type B hepatitis and infectious rhinotracheitis.

Key words: dairy cow; type B hepatitis; infectious rhinotracheitis; infectious pleuropneumonia