Abstract: The buttocks muscle can be increased about 30% by Callipyge (CLPG), resulting from an apparent A→G mutation in 32.8 kb upstream of GTL2 in the ovine chromosome 18. The mutation polymorphism is referred to as SNP CLPG, which was later shown to be the only difference between the callipyge and wild-type chromosome. The objective of the research is to select carriers of the callipyge gene by the molecular biological techniques, which is the basis of selection of the excellent meat traits. The SNPs were detected by PCR-restriction fragment length polymorphisms (PCR-RFLP) with one pairs of primers in genome of Dorset sheep, Texel sheep, Charolais sheep and Merino sheep. A→G substitution was found and one special products (493 bp) were amplified.

Key words: PCR-RFLP; Callipyge gene; SNP; sheep