猪流行性腹泻病毒重组腺病毒疫苗的构建及小鼠免疫试验

Abstract

Abstract: sM, M, S gene of PEDV vaccine stain were implified by amplified by RT-PCR and reorganization PCR, then inserted into the pShuttle-CMV vector. These recombinant shuttle plasmids were linearized by PmeⅠand then transformed to BJ5183 cells containing the pAdEasy-1 bone plasmid. The homologous recombinantplasmidwas transformed to XL-10 Gold ultracompetent cells in order to obtain this plasmid in a large scale. These recombinant adenoviruses were co-infected Vero cells, mice immune experiment indicated that antibodies were induced by Western blotting.

Key words: PEDV; recombinant adenovirus; vaccine; immunity

CLC Number:

S858.65

JIAO Mao-xing;WU Feng;LIU De-hui;HUANG Yu-mao. Construction of the PEDV Adenovirus Vaccine and Mice Immune Experiment[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(2): 11-15.

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Construction of the PEDV Adenovirus Vaccine and Mice Immune Experiment

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