China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (12): 166-169.

• 疾病防治 • Previous Articles     Next Articles

Establishment and Application of Transcription-Polymerase Chain Reaction for Identification of Field Isolate of Porcine Pseudorabies Virus and Vaccine Strain

DONG Hao1, WU Bo2,4, ZHANG Jian-ying3, HU Gui-xue4   

  1. 1. College of Life Science,Jilin Agricultural University, Changchun 130118,China;2. Heilongjiang Veterinary Institute,Qiqihaer 161006,China;3. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Shenzhen 518067,China;4. College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China
  • Received:2011-04-25 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

Abstract: On the base of gE and gB gene design two primers to distinguish vaccine strains and wild strains.Using this PCR method to detect PRV, PCV2, PPV, PEDV, PRRSV and only PRV could be detected.Using this method to make etiology survey in Jilin province, 32 cases were detected as pseudorabies rabies virus infection in 205 cases,the positive rate was 15.6%.

Key words: porcine pseudorabies; gE gene; gB gene

CLC Number: