猪伪狂犬病病毒gB蛋白表达及免疫原性分析

doi:10.16431/j.cnki.1671-7236.2017.04.033

Abstract

Abstract:

In order to study the expression and immunogenicity of porcine pseudorabies virus (PRV) gB protein,the specific primers were designed with the template of PRV preserved in the laboratory, and the 612 bp conserved gene fragments were amplified and sequenced, then it was cloned into the expression vector pET-28a and transformed into E.coli BL21 (DE3), the target protein was obtained after induced expression and purification.Western blotting was performed to analyse its immunogenicity. The results showed that gB protein was 30 ku, which mainly expressed in the form of inclusion body, and the concentration of the protein was 106 μg/mL, with well reactogenicity. 13 PRV positive serum and 16 negative serum in the samples were detected using ELISA Kit on sale, using positive serum, the PRV antibody detection method was initially established with the PRV gB protein as antigen package.

Key words: porcine pseudorabies virus; gB protein; prokaryotic expression; immunogenicity

CLC Number:

S852.65

JIA Gang, FAN Mei-na, GU Wei. Expression and Immunogenicity Analysis of Porcine Pseudorabies Virus gB Protein[J]. , 2017, 44(4): 1175-1181.

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Expression and Immunogenicity Analysis of Porcine Pseudorabies Virus gB Protein

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