›› 2011, Vol. 38 ›› Issue (12): 163-165.

• 疾病防治 • Previous Articles     Next Articles

Prevalence of Extended-Spectrum β-Lactamase in Escherichia coli Isolates from Food-producing Animals

ZHENG Hong-qing, DENG Yu-ting, HE Liang-ying, YANG Tong, YAO Qiong-fen, TIAN Wei, LIU Jian-hua    

  1. Guangdong Key Laboratory for Veterinary Drug Development and Safty Evaluation, College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China
  • Received:2011-04-18 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

Abstract: The purpose of this study was to investigate the prevalence of extended-spectrum β-lactamase(ESBLs) and CTX-M genes among Escherichia coli isolated from food-producing animals.A total of 1273 E. coli (707 from healthy animals and 566 from sick animals) isolated from food-producing animals,including pigs,chickens and ducks. ESBLs-producing isolates were screened by the double-disk synergy test. PCR was performed to amplify CTX-M genes among ESBL-producing isolates. ESBLs production was detected by the screening method for 181(14.2%) of the 1273 isolates. The percentage of ESBLs production in isolates from sick animals (19.9%) was higher than isolates from healthy animals (10.3%). In 181 ESBLs-producing strains,151 isolates (83.4%),representing 11.8% of the total E. coli isolates,harbored the CTX-M type β-lactamases. High prevalence of ESBL and CTM-M genes were found in E.coli isolates from food-producing animals. CTX-M genes were the main type of ESBLs in E.coli isolates from food-producing animals.

Key words: food-producing animal; Escherichia coli; ESBLs

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