蒙古羊羊水源干细胞分离培养及其成骨分化研究

›› 2011, Vol. 38 ›› Issue (11): 41-46.

• 生理生化 • Previous Articles Next Articles

Isolation and Culture of Amniotic Fluid-derived Stem Cells from Mongolian Sheep and Induction to Osteoblasts

HU Si-le¹, DA Lai², WU Jiang-hong¹, ZHOU Huan-min¹, ZHANG Jia-xin¹, RONG Wei-heng^1,2, ZHU Xian-guang², WANG Feng-wu²

1. College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018, China;2. Inner Mongolia Academy of Agriculture and Animal Husbandry,Hohhot 010031,China

Received:2011-03-24 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20

Abstract

Abstract: This study was designed to establish amniotic fluid-derived stem cells line and explore the potential to differentiate into osteoblasts. The amniotic fluid-derived stem cells were cultured under the condition of 15% FBS and detected the embryoid bodies forming ability and investigated the expressions of the stem cell markers (Oct-4,SH2,SSEA-1,Nanog,HLA-ABC,CD117) in different passage cell by RT-PCR. Furthermore,amniotic fluid-derived stem cells were induced by dexamethasone,β-glycerophosphate,ascorbic acid. Amniotic fluid-derived stem cells in vitro were able to expand extensively,form the embryoid body which expressed marker genes of three embryonic germ layers,and differentiate along osteoblasts. The results showed that we successfully isolated the Mongolian sheep amniotic fluid-derived stem cells and established cells line which can be induced to differentiate into osteoblasts.

Key words: amniotic fluid-derived stem cell; embryonic body; induction; osteoblasts

CLC Number:

S826.8⁺2

HU Si-le;DA Lai;WU Jiang-hong;ZHOU Huan-min;ZHANG Jia-xin;RONG Wei-heng;ZHU Xian-guang;WANG Feng-wu. Isolation and Culture of Amniotic Fluid-derived Stem Cells from Mongolian Sheep and Induction to Osteoblasts[J]. , 2011, 38(11): 41-46.

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Isolation and Culture of Amniotic Fluid-derived Stem Cells from Mongolian Sheep and Induction to Osteoblasts

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