盘羊脐带间充质干细胞的分离培养及诱导分化

doi:10.16431/j.cnki.1671-7236.2021.05.009

Abstract

Abstract: This experiment was aimed to study the isolation,culture,multi-lineage differentiation potential of umbilical cord mesenchymal stem cells (UC-MSCs) from argali in vitro.UC-MSCs were isolated from umbilical cord of newborn male fetus after delivery in argali by tissue block culture and cultured in vitro.UC-MSCs were induced to differentiate into osteoblasts,chondroblasts and adipoblasts to detect their multi-lineage differentiation potential.The results showed that the UC-MSCs of argali had the unique biological characteristics of being cluster and fibrous by tissue block culture,and the growth curve was "S" shape.The average doubling time of the cells was 33.50 h,and it could differentiate into osteoblasts,chondroblasts and adipoblasts.The osteoblasts were stained with Alizarin red S showing typical orange-red calcium deposits,chondroblasts were stained with Alcian blue showing a blue cartilage matrix,and adipoblasts were stained with oil red O showing dark red lipid droplets.Real-time quantitative PCR detection results revealed that with the extension of inducting time,the relative expression of bone gamma-carboxyglutamate protein (BGLAP),osteopontin (OPN) and Runt-related transcription factor 2 (RUNX2) genes in the osteogenic differentiation cells increased extremely significantly (P<0.01).The expression of lumican (LUM) gene in the chondrogenic differentiation cells increased significantly,while the expression of biglycan (BGN) and sex determining region Y-box 9 (SOX9) genes increased extremely significantly (P<0.01).The relative expression of lipoprotein lipase(LPL) and peroxisome proliferator-activated receptor γ(PPAR-γ) genes in the adipogenic differentiation cells increased extremely significantly (P<0.01).The results indicated that UC-MSCs could be isolated from umbilical cord tissue attached to placenta and differentiated into osteoblasts,chondroblasts and adipoblasts.

Key words: argali; umbilical cord mesenchymal stem cells; isolation and culture; in vitro differentiation induction

CLC Number:

Q254

ZHOU Guizhen, ZHOU Zhengna, TANG Xiaoyun, BI Jiaying, WANG Xuguang, LI Juan. Isolation,Culture and Induced Differentiation of Umbilical Cord Mesenchymal Stem Cells from Argali[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1584-1592.

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Isolation,Culture and Induced Differentiation of Umbilical Cord Mesenchymal Stem Cells from Argali

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