移植脐带间充质干细胞对荷斯坦公犊日增重及血清生长因子水平的影响

doi:10.16431/j.cnki.1671-7236.2015.01.011

Abstract

Abstract: This study was aimed to explore the effect on average daily weight gain and growth factor levels in the serum of Holstein bull calf after injecting umbilical cord mesenchymal stem cells (UC-MSCs).28 Holstein bull calves were selected with conditions of be born for about one month and similar weight, then they were divided into control and experimental groups.The test collected healthy fresh Holstein cow fetal umbilical cord organization, purificated and cultivated UC-MSCs in vitro by the method of pancreatic enzyme digestion.Calves of experimental group was injected in jugular vein with the successful UC-MSCs which diluted to 8 mL (3×10⁵ cells/kg), the control group was injected with saline.The test lasted for 154 d.The results showed that after injecting UC-MSCs, the average weight of experimental group was significantly higher than that of control group in 4, 5 and 6 months (P< 0.05), the average daily gain of experimental group was significantly higher than that of control group in 3 and 4 months (P< 0.05), while the difference was very significant in 6 months (P< 0.01).Serum insulin-like growth factor (IGF-1) level of experimental group was significantly higher than that of control group in 4 months (P< 0.05).Serum vascular endothelial growth factor (VEGF), transforming growth factor (TGF-α) levels of experimental group were significantly higher than that of control group in 3 months (P< 0.05).Serum VEGF, bFGF, TGF-α levels of experimental group were significantly or very significantly higher than that of control group (P< 0.05;P< 0.01).Serum BMP-7 level of experimental group was significantly lower than that of control group in 2, 3, 4 and 6 months (P< 0.05).In conclusion, UC-MSCs transplantation significantly improved male calf serum IGF-1, VEGF, TGF-α and bFGF levels, these growth factors and male calf serum in the growth and development were closely related, and then influence the growth performance.

Key words: umbilical cord mesenchymal stem cells; Holstein male calves; daily gain; growth factor

CLC Number:

S823

ZHAO Yan-kun, SHAO Wei, WANG Li-wen, PENG Guo-liang, ZHANG Xiao-xue, YU Xiong. Effect on Average Daily Weight Gain and Growth Factor Levels in the Serum of Holstein Bull Calf after Injecting Umbilical Cord Mesenchymal Stem Cells[J]. , 2015, 42(1): 74-79.

References

[1] Wollert K C,Drexler H.Clinical applications of stem cells for the heart[J].Circulation Research,2005,96(2):151-163.
[2] 邵伟.颈静脉注射脐带间充质干细胞对哈萨克羔羊生长发育及血清生化指标的影响[D].乌鲁木齐:新疆农业大学,2012.
[3] 秦小惠,古再丽努尔·艾麦提,邵伟,等.移植骨髓间充质干细胞对乳腺炎模型大鼠血清中酶及抗体水平的影响[J].中国畜牧兽医,2013,40(7):112-115.
[4] Lu L L,Liu Y J,Yang S G,et al.Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials[J].Haematologica,2006,91(8):1017-1026.
[5] Cao F J,Feng S Q.Human umbilical cord mesenchymal stem cells and the treatment of spinal cord injury [J].Chinical Medicine Journal,2009,122(2):225-231.
[6] Liechty K W,MacKenzie T C,Shaaban A F,et al.Human messenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheep[J].Nature Medicine,2000,6(11):1282-1286.
[7] 沈君,周翠屏,成丽娜,等.大鼠骨髓间充质干细胞的荧光双标记及MRI体外示踪的研究[J].中华放射学杂志,2008,42(4):426-431.
[8] 马晓伟,马芹颖,王彦永,等.骨髓间充质干细胞分泌的蛋白及其功能[J].中国组织工程研究与临床康复,2009,14:2763-2766.
[9] 姜丽红.胰岛素样生长因子和胰岛素样生长因子结合蛋白在人类胎儿生长中的作用[J].国外医学内分泌学分册,2002,1(22):49-50.
[10] Cianfarani S,Germani D,Rossi P,et al.Intrauterine growth retardation:Evidence for the activation of the insulin-like growth factor (IGF)-related growth-promoting machinery and the presence of a cation-independent IGF binding protein-3 proteolytic activity by two months of life[J].Pediatr Res,1998,44(3):374-380.
[11] 邢海权,闫梦菲.胰岛素样生长因子IGF-1的研究进展[J].畜禽业,2011,8(268):36-37.
[12] Markel T A,Wang Y,Herrmann J L,et al.VEGF is critical for stem cell mediated card in protection and a crucial paracrin e factor for defeining the age the reshold in adult and neonatal stem cell function[J].Am J Physiol Heart Circ Physiol,2008,295(6):2308-2314.
[13] 黄晨显,沈祖尧.血管内皮细胞生长因子和碱性成纤维细胞生长因子加速预构扩张皮瓣成熟的研究[J].中国修复重建外科杂志,2003,17:293-297.
[14] 高丽娜.生长因子联合组织工程对骨髓来源间充质干细胞促增殖作用的分子影像研究[D].西安:第四军医大学,2011.
[15] 王强,张斌,胡维昱,等.TGF-α和TGF-β1在胰腺癌组织中的表达及临床意义[J].中国现代普通外科进展,2012,13(5):360-361.
[16] Haaijman A,D'Souza R N,Bronckers A L,et al.OP-1 (BMP-7) affects mRNA expression of type Ⅰ,Ⅱ,Ⅹ collagen,and matrix Gla protein in ossifying long bones in vitro[J].J Bone Miner Res,1997,12(11):1815-1823.
[17] 金丹,裴国献,王珂,等.人骨形态发生蛋白7基因转染对骨髓间充质干细胞增殖及成骨分化性能的影响[J].中国临床康复,2005(14):206-208.
[18] Peng Y,Kang Q,Cheng H,et al.Transcriptional characterization of bone morphogenetic proteins (BMPs)-mediated osteogenic signaling[J].J Cell Biochem,2003,90(6):1149-1165.

Effect on Average Daily Weight Gain and Growth Factor Levels in the Serum of Holstein Bull Calf after Injecting Umbilical Cord Mesenchymal Stem Cells

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