Abstract: One strain virus(GD-09) was isolated from dead pigeon by SPF chicken embryos. This strain could agglutinate chicken erythrocytes and the heamagglutination of virus could be inhibited by Newcastle disease virus antiserum. It’s mean death time(MDT) of chicken embryos was 57.6 hours and its intracerebral pathogenicity index (ICPI) in one-day-old chicken was 1.74. That indicated it was a virulent strain. The cleavage site of the virus F protein was ¹¹²RRQRRF¹¹⁷,and accorded with highly pathogenic strain character. By analyzing genotype and homology of GD-09 and other NDV strains, it showed that GD-09 belonged to genotypeⅥ, and had lower homology compared with traditional strains La sota，F48E9，B1 and V4.

Key words: pigeon; Newcastle disease virus; F gene