线粒体在家畜卵母细胞成熟过程中的调控机制研究进展

doi:10.16431/j.cnki.1671-7236.2025.05.027

Abstract

Abstract: Oocytes develop and mature depending on the mitochondria to produce large amounts of adenosine triphosphate (ATP) to maintain transcription and translation necessary for maturation.In addition,mitochondria also carry the rate-limiting effect of steroid production.Therefore,mitochondrial dysfunction often leads to adverse pregnancy outcomes.The irrational environment,oxidative stress and other adverse factors in vitro culture medium are easy to damage oocyte mitochondria,resulting in the decrease of ATP and mitochondrial DNA (mtDNA) copy number,the increase of reactive oxygen species (ROS),the obstruction of spindle assembly,and the decrease of oocyte maturation rate and development potential.Studies have shown that exogenous substances such as melatonin and resveratrol can improve the mitochondrial function of oocytes and increase the maturation rate of oocytes.Although improving the quality of mitochondria can improve the production efficiency of domestic animal oocytes in vitro,the specific regulatory mechanism is still unclear.Therefore,it is necessary to investigate the mechanism of mitochondria in oocytes.The author briefly introduced the structure and function of mitochondria.Several mechanisms of mitochondrial regulation of oocyte maturation were reviewed,including signaling regulation of oocyte maturation,mtDNA and oocyte development,oocyte mitochondrial energetics and dynamics.The methods to improve oocyte mitochondria was proposed and its research prospect was prospected,aiming to provide reference for exploring the development ability of domestic animal oocytes through targeted improvement of mitochondrial function.

Key words: mitochondrion; oocyte; maturing rate; oxidative stress

CLC Number:

S814

GAO Zihao, LI Jia, ZHANG Linhui, ZHANG Ci, LIU Bingnan, LI Junjie, XIA Wei. Research Progress on the Regulatory Mechanisms of Mitochondria in the Maturation of Domestic Animal Oocytes[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 2232-2242.

References

[1] FERNANDEZ-VIZARRA E,ZEVIANI M.Mitochondrial disorders of the OXPHOS system[J].FEBS Letters,2021,595(8):1062-1106.
[2] KIRILLOVA A,SMITZ J E J,SUKHIKH G T,et al.The role of mitochondria in oocyte maturation[J].Cells,2021,10(9):2484.
[3] YILDIRIM R M, SELI E. The role of mitochondrial dynamics in oocyte and early embryo development[J]. Seminars in Cell & Developmental Biology, 2024, 159-160: 52-61.
[4] LI L,ZHU S,SHU W,et al.Characterization of metabolic patterns in mouse oocytes during meiotic maturation[J].Molecular Cell,2020,80(3):525-540.e9.
[5] PODOLAK A,WOCLAWEK-POTOCKA I,LUKASZUK K.The role of mitochondria in human fertility and early embryo development:What can we learn for clinical application of assessing and improving mitochondrial DNA?[J].Cells,2022,11(5):797.
[6] ADEBAYO M,SINGH S,SINGH A P,et al.Mitochondrial fusion and fission:The fine-tune balance for cellular homeostasis[J].FASEB Journal:Official Publication of the Federation of American Societies for Experimental Biology,2021,35(6):e21620.
[7] MURATA D,ARAI K,IIJIMA M,et al.Mitochondrial division,fusion and degradation[J].Journal of Biochemistry,2020,167(3):233-241.
[8] MOORE A S,COSCIA S M,SIMPSON C L,et al.Actin cables and comet tails organize mitochondrial networks in mitosis[J].Nature,2021,591(7851):659-664.
[9] DE VOS M,GRYNBERG M,HO T M,et al.Perspectives on the development and future of oocyte IVM in clinical practice[J].Journal of Assisted Reproduction and Genetics,2021,38(6):1265-1280.
[10] ADHIKARI D,LEE I W,YUEN W S,et al.Oocyte mitochondria—Key regulators of oocyte function and potential therapeutic targets for improving fertility[J].Biology of Reproduction,2022,106(2):366-377.
[11] BEN-MEIR A,BURSTEIN E,BORREGO-ALVAREZ A,et al.Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging[J].Aging Cell,2015,14(5):887-895.
[12] SUMEGI K,FEKETE K,ANTUS C,et al.BGP-15 protects against oxidative stress- or lipopolysaccharide-induced mitochondrial destabilization and reduces mitochondrial production of reactive oxygen species[J].PLoS One,2017,12(1):e0169372.
[13] GIACOMELLO M,PYAKUREL A,GLYTSOU C,et al.The cell biology of mitochondrial membrane dynamics[J].Nature Reviews.Molecular Cell Biology,2020,21(4):204-224.
[14] SKEIE J M,NISHIMURA D Y,WANG C L,et al.Mitophagy:An emerging target in ocular pathology[J].Investigative Ophthalmology & Visual Science,2021,62(3):22.
[15] BOYMAN L,KARBOWSKI M,LEDERER W J.Regulation of mitochondrial ATP production:Ca²⁺ signaling and quality control[J].Trends in Molecular Medicine,2020,26(1):21-39.
[16] SPINELLI J B,HAIGIS M C.The multifaceted contributions of mitochondria to cellular metabolism[J].Nature Cell Biology,2018,20(7):745-754.
[17] VAN BLERKOM J.Mitochondrial function in the human oocyte and embryo and their role in developmental competence[J].Mitochondrion,2011,11(5):797-813.
[18] MITCHELL P.Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism[J].Nature,1961,191(4784):144-148.
[19] MILLER W L.Disorders in the initial steps of steroid hormone synthesis[J].The Journal of Steroid Biochemistry and Molecular Biology,2017,165(Pt A):18-37.
[20] MILLER W L.Steroid hormone synthesis in mitochondria[J].Molecular and Cellular Endocrinology,2013,379(1-2):62-73.
[21] NEBERT D W,WIKVALL K,MILLER W L.Human cytochromes P450 in health and disease[J].Philosophical Transactions of the Royal Society B:Biological Sciences,2013，368(1612):20120431.
[22] ALLEN J A,SHANKARA T,JANUS P,et al.Energized,polarized,and actively respiring mitochondria are required for acute leydig cell steroidogenesis[J].Endocrinology,2006,147(8):3924-3935.
[23] ARTEMENKO I P,ZHAO D,HALES D B,et al.Mitochondrial processing of newly synthesized steroidogenic acute regulatory protein (StAR),but not total StAR,mediates cholesterol transfer to cytochrome P450 side chain cleavage enzyme in adrenal cells[J].Journal of Biological Chemistry,2001,276(49):46583-46596.
[24] LEQUARRE A S,VIGNERON C,RIBAUCOUR F,et al.Influence of antral follicle size on oocyte characteristics and embryo development in the bovine[J].Theriogenology,2005,63(3):841-859.
[25] OGUSHI S,PALMIERI C,FULKA H,et al.The maternal nucleolus is essential for early embryonic development in mammals[J].Science,2008,319(5863):613-616.
[26] ZHANG M,XIA G.Hormonal control of mammalian oocyte meiosis at diplotene stage[J].Cellular and Molecular Life Sciences,2012,69(8):1279-1288.
[27] XI G,AN L,JIA Z,et al.Natriuretic peptide receptor 2(NPR2) localized in bovine oocyte underlies a unique mechanism for C-type natriuretic peptide (CNP)-induced meiotic arrest[J].Theriogenology,2018,106:198-209.
[28] STRCZY AN＇G SKA P,PAPIS K,MORAWIEC E,et al.Signaling mechanisms and their regulation during in vivo or in vitro maturation of mammalian oocytes[J].Reproductive Biology and Endocrinology,2022,20(1):37.
[29] HAO X,WANG Y,KONG N,et al.Epidermal growth factor-mobilized intracellular calcium of cumulus cells decreases natriuretic peptide receptor 2 affinity for natriuretic peptide type C and induces oocyte meiotic resumption in the mouse[J].Biology of Reproduction,2016,95(2):45.
[30] EPPIG J J.Coordination of nuclear and cytoplasmic oocyte maturation in eutherian mammals[J].Reproduction,Fertility,and Development,1996,8(4):485-489.
[31] HILL J H,CHEN Z,XU H.Selective propagation of functional mitochondrial DNA during oogenesis restricts the transmission of a deleterious mitochondrial variant[J].Nature Genetics,2014,46(4):389-392.
[32] HARMAN D.Aging:A theory based on free radical and radiation chemistry[J].Journal of Gerontology,1956,11(3):298-300.
[33] JANSEN R.The bottleneck:Mitochondrial imperatives in oogenesis and ovarian follicular fate[J].Molecular and Cellular Endocrinology,145(1-2):81-88.
[34] WEI Y H,LEE H C.Oxidative stress,mitochondrial DNA mutation,and impairment of antioxidant enzymes in aging[J].Experimental Biology and Medicine,2002,227(9):671-682.
[35] WARZYCH E,LIPINSKA P.Energy metabolism of follicular environment during oocyte growth and maturation[J].The Journal of Reproduction and Development,2020,66(1):1-7.
[36] DELL’AQUILA M E,AMBRUOSI B,DE SANTIS T,et al.Mitochondrial distribution and activity in human mature oocytes:Gonadotropin-releasing hormone agonist versus antagonist for pituitary down-regulation[J].Fertility and Sterility,2009,91(1):249-255.
[37] SIRARD M A.Distribution and dynamics of mitochondrial DNA methylation in oocytes,embryos and granulosa cells[J].Scientific Reports,2019,9(1):11937.
[38] LONG S,ZHENG Y,DENG X,et al.Maintaining mitochondrial DNA copy number mitigates ROS-induced oocyte decline and female reproductive aging[J].Communications Biology,2024,7(1):1229.
[39] DALTON C M,SZABADKAI G,CARROLL J.Measurement of ATP in single oocytes:Impact of maturation and cumulus cells on levels and consumption[J].Journal of Cellular Physiology,2014,229(3):353-361.
[40] RICHANI D,LAVEA C F,KANAKKAPARAMBIL R,et al.Participation of the adenosine salvage pathway and cyclic AMP modulation in oocyte energy metabolism[J].Scientific Reports,2019,9(1):18395.
[41] RICHANI D,GILCHRIST R B.Approaches to oocyte meiotic arrest in vitro and impact on oocyte developmental competence[J].Biology of Reproduction,2022,106(2):243-252.
[42] LOHKA M J,MALLER J L.Induction of nuclear envelope breakdown,chromosome condensation,and spindle formation in cell-free extracts[J].Journal of Cell Biology,1985,101(2):518-523.
[43] WU M,GERHART J C.Partial purification and characterization of the maturation-promoting factor from eggs of Xenopus laevis[J].Developmental Biology,1980,79(2):465-477.
[44] CYERT M S,KIRSCHNER M W.Regulation of MPF activity in vitro[J].Cell,1988,53(2):185-195.
[45] JAFFE L A,EGBERT J R.Regulation of mammalian oocyte meiosis by intercellular communication within the ovarian follicle[J].Annual Review of Physiology,2017,79:237-260.
[46] FAIENZA F,POLVERINO F,RAJENDRAPRASAD G,et al.AMBRA1 phosphorylation by CDK1 and PLK1 regulates mitotic spindle orientation[J].Cellular and Molecular Life Sciences,2023,80(9):251.
[47] VAN BLERKOM J,DAVIS P W,LEE J.ATP content of human oocytes and developmental potential and outcome after in-vitro fertilization and embryo transfer[J].Human Reproduction,1995,10(2):415-424.
[48] DUMOLLARD R,DUCHEN M,CARROLL J.The role of mitochondrial function in the oocyte and embryo[J].Current Topics in Developmental Biology,2007,77:21-49.
[49] DUMOLLARD R,MARANGOS P,FITZHARRIS G,et al.Sperm-triggered Ca²⁺ oscillations and Ca²⁺ homeostasis in the mouse egg have an absolute requirement for mitochondrial ATP production[J].Development,2004,131(13):3057-3067.
[50] FONTANA J,MARTÍNKOVÁ S,PETR J,et al.Metabolic cooperation in the ovarian follicle[J].Physiological Research,2020,69(1):33-48.
[51] WANG H,CAI H,WANG X,et al.HDAC3 maintains oocyte meiosis arrest by repressing amphiregulin expression before the LH surge[J].Nature Communications,2019,10(1):5719.
[52] LIU X,XIE F,ZAMAH A M,et al.Multiple pathways mediate luteinizing hormone regulation of cGMP signaling in the mouse ovarian follicle[J].Biology of Reproduction,2014,91(1):9.
[53] MAY-PANLOUP P,CHRÉTIEN M F,JACQUES C,et al.Low oocyte mitochondrial DNA content in ovarian insufficiency[J].Human Reproduction,2005,20(3):593-597.
[54] MA C,XU Y,ZHANG X,et al.Melatonin mitigates PNMC-induced disruption of spindle assembly and mitochondrial function in mouse oocytes[J].Ecotoxicology and Environmental Safety,2024,282:116703.
[55] BABAYEV E,SELI E.Oocyte mitochondrial function and reproduction[J].Current Opinion in Obstetrics & Gynecology,2015,27(3):175.
[56] BAHETY D,BÖKE E,RODRÍGUEZ-NUEVO A.Mitochondrial morphology,distribution and activity during oocyte development[J].Trends in Endocrinology and Metabolism,2024,35(10):902-917.
[57] RUAN W,LIM H H,SURANA U.Mapping mitotic death:Functional integration of mitochondria,spindle assembly checkpoint and apoptosis[J].Frontiers in Cell and Developmental Biology,2018,6:177.
[58] WANG Q,LEADER A,TSANG B K.Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells[J].Journal of Ovarian Research,2013,6(1):23.
[59] BAENA V,TERASAKI M.Three-dimensional organization of transzonal projections and other cytoplasmic extensions in the mouse ovarian follicle[J].Scientific Reports,2019,9(1):1262.
[60] PICCA A,MANKOWSKI R T,BURMAN J L,et al.Mitochondrial quality control mechanisms as molecular targets in cardiac ageing[J].Nature Reviews Cardiology,2018,15(9):543-554.
[61] WAKAI T,HARADA Y,MIYADO K,et al.Mitochondrial dynamics controlled by mitofusins define organelle positioning and movement during mouse oocyte maturation[J].Molecular Human Reproduction,2014,20(11):1090-1100.
[62] UDAGAWA O,ISHIHARA T,MAEDA M,et al.Mitochondrial fission factor Drp1 maintains oocyte quality via dynamic rearrangement of multiple organelles[J].Current Biology,2014,24(20):2451-2458.
[63] LI X,STRAUB J,MEDEIROS T C,et al.Mitochondria shed their outer membrane in response to infection-induced stress[J].Science,2022,375(6577):eabi4343.
[64] ADHIKARI D,LEE I W,AL-ZUBAIDI U,et al.Depletion of oocyte dynamin-related protein 1 shows maternal-effect abnormalities in embryonic development[J].Science Advances,2022,8(24):eabl8070.
[65] VANTAGGIATO C,CASTELLI M,GIOVARELLI M,et al.The fine tuning of Drp1-dependent mitochondrial remodeling and autophagy controls neuronal differentiation[J].Frontiers in Cellular Neuroscience,2019,13：120.
[66] MOORE B A,GONZALEZ AVILES G D,LARKINS C E,et al.Mitochondrial retention of Opa1 is required for mouse embryogenesis[J].Mammalian Genome,2010,21(7):350-360.
[67] CHIARATTI M R.Uncovering the important role of mitochondrial dynamics in oogenesis:Impact on fertility and metabolic disorder transmission[J].Biophysical Reviews,2021,13(6):967-981.
[68] ZHANG M,BENER M B,JIANG Z,et al.Mitofusin 1 is required for female fertility and to maintain ovarian follicular reserve[J].Cell Death & Disease,2019,10(8):560.
[69] ZHANG M,BENER M B,JIANG Z,et al.Mitofusin 2 plays a role in oocyte and follicle development,and is required to maintain ovarian follicular reserve during reproductive aging[J].Aging,2019,11(12):3919-3938.
[70] PARK M R,HWANG I S,KWAK T U,et al.Low expression of mitofusin 1 is associated with mitochondrial dysfunction and apoptosis in porcine somatic cell nuclear transfer embryos[J].Animal Science Journal,2020,91(1):e13430.
[71] WU S,ZHAO X,WU M,et al.Low expression of mitofusin 1 gene leads to mitochondrial dysfunction and embryonic genome activation failure in ovine-bovine inter-species cloned embryos[J].International Journal of Molecular Sciences,2022,23(17):10145.
[72] HOU X,ZHU S,ZHANG H,et al.Mitofusin1 in oocyte is essential for female fertility[J].Redox Biology,2019,21:101110.
[73] HUA S,ZHANG H,SONG Y,et al.High expression of Mfn1 promotes early development of bovine SCNT embryos:Improvement of mitochondrial membrane potential and oxidative metabolism[J].Mitochondrion,2012,12(2):320-327.
[74] ZHANG H,LI C,WEN D,et al.Melatonin improves the quality of maternally aged oocytes by maintaining intercellular communication and antioxidant metabolite supply[J].Redox Biology,2022,49:102215.
[75] KANG J T,KOO O J,KWON D K,et al.Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells[J].Journal of Pineal Research,2009,46(1):22-28.
[76] PARK D,JEONG H,LEE M N,et al.Resveratrol induces autophagy by directly inhibiting mTOR through ATP competition[J].Scientific Reports,2016,6:21772.
[77] LI Y,WANG J,ZHANG Z,et al.Resveratrol compares with melatonin in improving in vitro porcine oocyte maturation under heat stress[J].Journal of Animal Science and Biotechnology,2016,7(1):33.
[78] SUGIYAMA M,KAWAHARA-MIKI R,KAWANA H,et al.Resveratrol-induced mitochondrial synthesis and autophagy in oocytes derived from early antral follicles of aged cows[J].The Journal of Reproduction and Development,2015,61(4):251-259.
[79] LIU M J,SUN A G,ZHAO S G,et al.Resveratrol improves in vitro maturation of oocytes in aged mice and humans[J].Fertility and Sterility,2018,109(5):900-907.
[80] CAO Y,ZHAO H,WANG Z,et al.Quercetin promotes in vitro maturation of oocytes from humans and aged mice[J].Cell Death & Disease,2020,11(11):965.
[81] WANG H,JO Y J,OH J S,et al.Quercetin delays postovulatory aging of mouse oocytes by regulating SIRT expression and MPF activity[J].Oncotarget,2017,8(24):38631-38641.
[82] SÁ N A R,VIEIRA L A,FERREIRA A C A,et al.Anethole supplementation during oocyte maturation improves in vitro production of bovine embryos[J].Reproductive Sciences,2019，26:1933719119831783.
[83] NIE X,DONG X,HU Y,et al.Coenzyme Q10 stimulate reproductive vatality[J].Drug Design,Development and Therapy,2023,17:2623-2637.
[84] BEN-MEIR A,BURSTEIN E,BORREGO-ALVAREZ A,et al.Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging[J].Aging Cell,2015,14(5):887-895.
[85] MA L,CAI L,HU M,et al.Coenzyme Q10 supplementation of human oocyte in vitro maturation reduces postmeiotic aneuploidies[J].Fertility and Sterility,2020,114(2):331-337.
[86] YANG C X,LIU S,MIAO J K,et al.CoQ10 improves meiotic maturation of pig oocytes through enhancing mitochondrial function and suppressing oxidative stress[J].Theriogenology,2021,159:77-86.
[87] PIERRE C J,AZEEZ T A,ROSSETTI M L,et al.Long-term administration of resveratrol and MitoQ stimulates cavernosum antioxidant gene expression in a mouse castration model of erectile dysfunction[J]. Life Sciences,2022,310:121082.
[88] AL-ZUBAIDI U,ADHIKARI D,CINAR O,et al.Mitochondria-targeted therapeutics,MitoQ and BGP-15,reverse aging-associated meiotic spindle defects in mouse and human oocytes[J].Human Reproduction ,2021,36(3):771-784.
[89] KELSO G F,PORTEOUS C M,COULTER C V,et al.Selective targeting of a redox-active ubiquinone to mitochondria within cells:Antioxidant and antiapoptotic properties[J].The Journal of Biological Chemistry,2001,276(7):4588-4596.
[90] SHARMA M,PUNETHA M,SAINI S,et al.Mito-Q supplementation of in vitro maturation or in vitro culture medium improves maturation of buffalo oocytes and developmental competence of cloned embryos by reducing ROS production[J].Animal Reproduction Science,2024,260:107382.

Research Progress on the Regulatory Mechanisms of Mitochondria in the Maturation of Domestic Animal Oocytes

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