外源添加猪胆酸对高脂孕鼠体脂代谢、肠道微生物和胆汁酸代谢的影响

doi:10.16431/j.cnki.1671-7236.2025.05.006

Abstract

Abstract: 【Objective】 This study was aimed to investigate the effects of adding hyocholic acid (HCA) on body lipid metabolism,intestinal microorganisms and bile acid metabolism in high-fat pregnant mice,and investigate the effects of HCA on improving the disorders of body lipid metabolism in mice.【Method】 Sixty-eight female and twenty-three male C57BL/6 mice with similar body weights(19.2 g±0.1 g) and good growth conditions were selected at 6 weeks of age to mate,the pregnant mice were randomly divided into four treatment groups based on their body weight:Control group (CON),high-fat group (T1),high-fat+50 mg/kg HCA group (T2) and high-fat+100 mg/kg HCA group (T3),with five replicates in each group and three mice per replicate.On the day of delivery and weaning,the growth performance,serum biochemical indices,and tissue section analysis of mice were performed.On the day of delivery,colon contents were collected for analysis of gut microbiota and bile acid metabolism.【Result】 ①Compared with CON group,the average weaning litter weight of mice was significantly increased in T1,T2 and T3 groups (P＜0.05),the subcutaneous fat weight of mice was significantly increased in T1 and T3 groups (P＜0.05),and the liver weight was significantly increased in T1 group (P＜0.05).②HCA could improve fat accumulation in liver and increase the amount of brown fat in scapular region of mice induced by high-fat diet.③Compared with CON group,the levels of total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) in serum of mice after delivery in T1,T2 and T3 groups were significantly increased (P＜0.05),the level of triglycerides (TG) in serum of mice in T1 and T3 groups was significantly increased (P＜0.05).After weaning,the levels of TC and HDL-C in serum of mice in T1,T2 and T3 groups were significantly increased (P＜0.05),the level of low-density lipoprotein cholesterol (LDL-C) in serum of mice in T1 and T2 groups was significantly increased (P＜0.05).④Alpha diversity analysis results showed that compared with CON group,Shannon and Simpson indexes of intestinal microflora of mice in T3 group were significantly decreased (P＜0.05),but Dominance index was significantly increased (P＜0.05).⑤At the phylum level,the first and second dominant bacteria in intestine of mice in each group were Bacteroidetes and Firmicutes,respectively.The third dominant bacteria in intestine of mice in T3 group was Desulfobacterota,and in other groups was Verrucomicrobiota.⑥Compared with CON group,the relative abundances of Lactobacillus,Clostridium and Bifidobacterium in intestine of mice in T1 and T2 groups were significantly increased (P＜0.05),the relative abundance of Coriobacterium in T3 group was significantly increased (P＜0.05),and the relative abundance of Bacteroides in T1,T2 and T3 groups was significantly decreased (P＜0.05).⑦Compared with CON group,the levels of 7-ketoLCA,alpha-MCA,beta-MCA and GLCA in intestine of mice in T1,T2 and T3 groups was significantly increased (P＜0.05),the levels of beta-UDCA and THCA in T2 and T3 groups were significantly increased (P＜0.05),and the level of 7,12-diketoLCA in T2 group was significantly increased (P＜0.05).【Conclusion】 HCA could improve the serum biochemical indices and promote the generation of beneficial bacteria and bile acid metabolism,thereby regulating the body fat metabolism disorder induced by high-fat diet in mice,providing a reference for the role of HCA in improving body lipid metabolism disorders under high-fat diet.

Key words: mice; hyocholic acid; high-fat diet; lipid metabolism; intestinal microorganisms; bile acid metabolism

CLC Number:

S816.7

CAO Chang, LI Yulian, WANG Jie, HE Qing, GONG Yanmei, FAN Zhiyong. Effects of Adding Hyocholic Acid on Body Lipid Metabolism,Intestinal Microorganisms and Bile Acid Metabolism in High-fat Pregnant Mice[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 1999-2011.

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Effects of Adding Hyocholic Acid on Body Lipid Metabolism,Intestinal Microorganisms and Bile Acid Metabolism in High-fat Pregnant Mice

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