饲粮中添加超氧化物歧化酶对肉鸡生长性能、免疫性能、抗氧化能力和肠道功能的影响

doi:10.16431/j.cnki.1671-7236.2025.05.005

Abstract

Abstract: 【Objective】 This experiment was conducted to explore the effects of dietary superoxide dismutase (SOD) on growth performance,immune performance,antioxidant capacity and intestinal function of broilers,so as to provide reference for the application of SOD in livestock and poultry.【Method】 A total of 300 one-day-old Arbor Acres (AA) male broilers were randomly divided into 5 groups with 6 replicates per group and 10 broilers per replicate.Broilers in control group were fed the basal diet,and broilers in groups A,B,C and D were fed the basal diet supplemented with 100,200,400 and 800 mg/kg SOD,respectively.The trial period was 42 days.The feed intake of broilers was recorded every day.At the ending of the feeding trial,one broiler was randomly selected from each replicate,and weighed after 12 h of water supply and fasting.The immune organs,blood,liver and intestinal samples were collected to determine the growth performance,immune performance,antioxidant capacity and intestinal function indexes of broilers.【Result】 Compared with control group,①The feed to gain (F/G) of broilers aged 1-21 days in group B was significantly decreased (P＜0.05).②The thymus index and bursa of Fabricius index of broilers in groups A-D showed an increasing trend,but the difference was not significant (P＞0.05).The spleen index of broilers in groups B and C was significantly increased (P＜0.05).③There was no significant difference in serum immunoglobulin A (IgA) content of broilers in groups A-D (P＞0.05),but the contents of IgG and IgM in serum of broilers in group B were significantly increased (P＜0.05).④The total antioxidant capacity (T-AOC),glutathione peroxidase (GSH-Px) and total superoxide dismutase (SOD) in serum,and T-AOC and SOD activities in liver of broilers in groups B and C were significantly increased (P＜0.05),and the malondialdehyde (MDA) content in serum and liver was extremely significantly decreased (P＜0.01).The activities of GSH-Px and catalase (CAT) in liver of broilers in group B were significantly increased (P＜0.05).The MDA content in serum of broilers in group D was significantly increased (P＜0.05).⑤The length of duodenum and jejunum and the weight of duodenum of broilers in group B were significantly increased (P＜0.05).The trypsin activity in duodenum and jejunum of broilers in groups B and C were significantly increased (P＜0.05).【Conclusion】 Under the conditions of this experiment,adding 200 mg/kg SOD to diet could improve the antioxidant capacity,immunity,intestinal function and 1-21 days old growth performance of broilers.

Key words: superoxide dismutase; broilers; growth performance; immune function; antioxidant capacity; intestinal function

CLC Number:

S816.73

ZHANG Guanfeng, SONG Xianfan, YAN Zhaoming, YANG Xiaojin, ZHENG Mengli, LIU Yating, CHEN Qinghua. Effects of Dietary Superoxide Dismutase Supplementation on Growth Performance, Immune Performance,Antioxidant Capacity and Intestinal Function of Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 1987-1998.

References

[1] ZELKO I N,MARIANI T J,FOLZ R J.Superoxide dismutase multigene family:A comparison of the CuZn-SOD (SOD1),Mn-SOD (SOD2),and EC-SOD (SOD3) gene structures,evolution,and expression[J].Free Radical Biology and Medicine,2002,33(3):337-349.
[2] WANG Y,BRANICKY R,NOË A,et al.Superoxide dismutases:Dual roles in controlling ROS damage and regulating ROS signaling[J].Journal of Cell Biology,2018,217(6):1915-1928.
[3] ZHENG M,LIU Y,ZHANG G,et al.The applications and mechanisms of superoxide dismutase in medicine,food,and cosmetics[J].Antioxidants,2023,12(9):1675.
[4] KAUR N,K SHARMA A,SHAKEEL A,et al.Therapeutic implications of superoxide dismutase and its importance in kinase drug discovery[J]. Current Topics in Medicinal Chemistry,2017,17(22):2495-2508.
[5] YOUNUS H.Therapeutic potentials of superoxide dismutase[J].International Journal of Health Sciences,2018,12(3):88.
[6] LIU M,SUN X,CHEN B,et al.Insights into manganese superoxide dismutase and human diseases[J].International Journal of Molecular Sciences,2022,23(24):15893.
[7] WARNER H R.Superoxide dismutase,aging,and degenerative disease[J].Free Radical Biology and Medicine,1994,17(3):249-258.
[8] NGUYEN N H,TRAN G B,NGUYEN C T.Anti-oxidative effects of superoxide dismutase 3 on inflammatory diseases[J].Journal of Molecular Medicine,2020,98(1):59-69.
[9] DONG L,CHEN Y,GU L,et al.Oral delivery of a highly stable superoxide dismutase as a skin aging inhibitor[J].Biomedicine & Pharmacotherapy,2023,164:114878.
[10] ISLAM M N,RAUF A,FAHAD F I,et al.Superoxide dismutase:An updated review on its health benefits and industrial applications[J].Critical Reviews in Food Science and Nutrition,2022,62(26):7282-7300.
[11] ZAKOŠEK PIPAN M,MRKUN J,KOSEC M,et al.Superoxide dismutase:A predicting factor for boar semen characteristics for short-term preservation[J]. BioMed Research International,2014,2014:105280.
[12] PAPAS M,CATALAN J,BARRANCO I,et al.Total and specific activities of superoxide dismutase (SOD) in seminal plasma are related with the cryotolerance of jackass spermatozoa[J].Cryobiology,2020,92:109-116.
[13] BAFANA A,DUTT S,KUMAR S,et al.Superoxide dismutase:An industrial perspective[J]. Critical Reviews in Biotechnology,2011,31(1):65-76.
[14] DONG X,WANG W,JIANG T,et al.Construction and potential application of bacterial superoxide dismutase expressed in Bacillus subtilis against mycotoxins[J].PLoS One,2021,16(11):e0260047.
[15] ZHAO Y,ZHAO L,ZHANG W,et al.Production of a novel superoxide dismutase by Escherichia coli and Pichia pastoris and analysis of the thermal stability of the enzyme[J].Frontiers in Nutrition,2022,9:850824.
[16] WLAZLAK S,PIETRZAK E,BIESEK J,et al.Modulation of the immune system of chickens a key factor in maintaining poultry production—A review[J].Poultry Science,2023，102(8):102785.
[17] BASIOUNI S,TELLEZ-ISAIAS G,LATORRE J D,et al.Anti-inflammatory and antioxidative phytogenic substances against secret killers in poultry:Current status and prospects[J]. Veterinary Sciences,2023,10(1):55.
[18] MIAO Z Q,DONG Y Y,QIN X,et al.Dietary supplementation of methionine mitigates oxidative stress in broilers under high stocking density[J]. Poultry Science,2021,100(8):101231.
[19] CHEN J,CHEN K,YUAN S,et al.Effects of aflatoxin B₁ on oxidative stress markers and apoptosis of spleens in broilers[J]. Toxicology and Industrial Health,2016,32(2):278-284.
[20] QUINTEIRO-FILHO W M,RIBEIRO A,FERRAZ-DE-PAULA V,et al.Heat stress impairs performance parameters,induces intestinal injury,and decreases macrophage activity in broiler chickens[J].Poultry Science,2010,89(9):1905-1914.
[21] WU B,CUI H,PENG X,et al.Dietary nickel chloride induces oxidative intestinal damage in broilers[J].International Journal of Environmental Research and Public Health,2013,10(6):2109-2119.
[22] ROSA A C,CORSI D,CAVI N,et al.Superoxide dismutase administration:A review of proposed human uses[J].Molecules,2021,26(7):1844.
[23] HE L,HE T,FARRAR S,et al.Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species[J].Cellular Physiology and Biochemistry,2017,44(2):532-553.
[24] BRENNEISEN P,STEINBRENNER H,SIES H.Selenium,oxidative stress,and health aspects[J].Molecular Aspects of Medicine,2005,26(4-5):256-267.
[25] MATHERS J,FRASER J A,MCMAHON M,et al.Antioxidant and cytoprotective responses to redox stress[J].Biochemical Society Symposium,2004,71:157-176.
[26] SIES H.Role of reactive oxygen species in biological processes[J].Klinische Wochenschrift,1991,69:965-968.
[27] LAURIDSEN C.From oxidative stress to inflammation:Redox balance and immune system[J].Poultry Science,2019,98(10):4240-4246.
[28] BHATTACHARYYA A,CHATTOPADHYAY R,MITRA S,et al.Oxidative stress:An essential factor in the pathogenesis of gastrointestinal mucosal diseases[J]. Physiological Reviews,2014,94(2):329-354.
[29] MOURAD F H,SAADÉ N E.Neural regulation of intestinal nutrient absorption[J].Progress in Neurobiology,2011,95(2):149-162.
[30] WONG C H,HAQUE M A,CHANG H C.Superoxide dismutase SOD-3 regulates redox homeostasis in the intestine[J]. Micropublication Biology,2023,2023:10.17912.
[31] LE H H,ZHAO W,FURNESS J B,et al.Using recombinant superoxide dismutase to control oxidative stress in the gastrointestinal tract of cyclic heat-stressed pigs[J]. Animals,2023,13(16):2681.
[32] TANG M,FANG R,XUE J,et al.Effects of catalase on growth performance,antioxidant capacity,intestinal morphology,and microbial composition in Yellow broilers[J].Frontiers in Veterinary Science,2022,9:802051.
[33] WU S,LI T,NIU H,et al.Effects of glucose oxidase on growth performance,gut function,and cecal microbiota of broiler chickens[J]. Poultry Science,2019,98(2):828-841.
[34] 马渭青,王思博,杨季,等.饲粮中添加超氧化物歧化酶模拟物对肉鸡生长性能,血清免疫指标及肠道抗氧化指标和消化酶活性的影响[J].动物营养学报,2020,32(1):432-439.MA W Q,WANG S B,YANG J,et al.Effects of dietary superoxide dismutase mimics on growth performance,serum immune indexes and intestinal antioxidant indexes and digestive enzyme activities of broilers[J].Chinese Journal of Animal Nutrition,2020,32(1):432-439.(in Chinese)
[35] MICHIELS C,RAES M,TOUSSAINT O,et al.Importance of Se-glutathione peroxidase,catalase,and Cu/Zn-SOD for cell survival against oxidative stress[J].Free radical Biology and Medicine,1994,17(3):235-248.
[36] TANG D,WU J,JIAO H,et al.The development of antioxidant system in the intestinal tract of broiler chickens[J].Poultry Science,2019,98(2):664-678.
[37] HE W,TIAN L,FANG F,et al.Limited hydrolysis and conjugation of zein with chitosan oligosaccharide by enzymatic reaction to improve functional properties[J].Food Chemistry,2021,348:129035.
[38] LI H,BYRNE K,HOWITT C A,et al.Efficient extraction and digestion of gluten proteins[J]. Functional Proteomics:Methods and Protocols,2019,1871:405-412.
[39] WEBER E,EHRLEIN H J.Reserve capacities of the small intestine for absorption of energy[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,1998,275(1):R300-R307.
[40] AL-KHALAIFAH H,AL-NASSER A.Dietary supplementation with various fat oils affect phytohemagglutinin skin test in broiler chickens[J]. Frontiers in Immunology,2020,11:1735.
[41] 陆牡龙,黄恩媛,陶秋妍,等.日粮中添加菊粉和鱼腥草对肉鸡生长性能,器官指数及肠道生长发育的影响[J].国外畜牧学(猪与禽),2021,41(3):78-85.LU M L,HUANG E Y,TAO Q Y,et al.Effects of dietary inulin and Houttuynia cordata on growth performance,organ index and intestinal growth and development of broilers[J].Animal Science Abroad(Pigs and Poultry),2021,41(3):78-85.(in Chinese)
[42] PEKMEZCI D.Vitamin E and immunity[J].Vitamins & Hormones,2011,86:179-215.
[43] SUN S,LI B,WU M,et al.Effect of dietary supplemental vitamin C and betaine on the growth performance,humoral immunity,immune organ index,and antioxidant status of broilers under heat stress[J].Tropical Animal Health and Production,2023,55(2):96.
[44] WU Q J,ZHENG X C,WANG T,et al.Effects of dietary supplementation with oridonin on the growth performance,relative organ weight,lymphocyte proliferation,and cytokine concentration in broiler chickens[J].BMC Veterinary Research,2018,14(1):34.
[45] LEWIS E D,MEYDANI S N,WU D.Regulatory role of vitamin E in the immune system and inflammation[J].IUBMB Life,2019,71(4):487-494.
[46] YASUMA R,CICATIELLO V,MIZUTANI T,et al.Intravenous immune globulin suppresses angiogenesis in mice and humans[J]. Signal Transduction and Targeted Therapy,2016,1:15002.
[47] DE SOUSA-PEREIRA P,WOOF J M.IgA:Structure,function,and developability[J].Antibodies,2019,8(4):57.
[48] BUNKER J J,ERICKSON S A,FLYNN T M,et al.Natural polyreactive IgA antibodies coat the intestinal microbiota[J].Science,2017,358(6361):eaan6619.
[49] EHRENSTEIN M R,COOK H T,NEUBERGER M S.Deficiency in serum immunoglobulin IgM predisposes to development of IgG autoantibodies[J]. The Journal of Experimental Medicine,2000,191(7):1253-1258.
[50] 王洪军.葡萄糖氧化酶和过氧化氢酶对肉鸡生长性能及免疫性能的影响[D].沈阳：沈阳农业大学，2019.WANG H J.Effects of glucose oxdase and catalase on production performance and immune performance in broilers[D].Shenyang:Shenyang Agricultural University,2019.(in Chinese)
[51] WANG S,ZHANG L,LI J,et al.Effects of dietary marigold extract supplementation on growth performance,pigmentation,antioxidant capacity and meat quality in broiler chickens[J].Asian-Australasian Journal of Animal Sciences,2017,30(1):71.
[52] YANG R,LI W,SHI Y H,et al.Lipoic acid prevents high-fat diet-induced dyslipidemia and oxidative stress:A microarray analysis[J]. Nutrition,2008,24(6):582-588.
[53] KUBES P,JENNE C.Immune responses in the liver[J].Annual Review of Immunology,2018,36:247-277.
[54] GEBHARDT R.Metabolic zonation of the liver:Regulation and implications for liver function[J].Pharmacology & Therapeutics,1992,53(3):275-354.
[55] CESARATTO L,VASCOTTO C,CALLIGARIS S,et al.The importance of redox state in liver damage[J].Annals of Hepatology,2004,3(3):86-92.
[56] ZHAO W,HUANG Y,CUI N,et al.Glucose oxidase as an alternative to antibiotic growth promoters improves the immunity function,antioxidative status,and cecal microbiota environment in White-feathered broilers[J]. Frontiers in Microbiology,2023,14:1100465.
[57] CHEN L,LIU Y,ZHANG Y,et al.Superoxide dismutase ameliorates oxidative stress and regulates liver transcriptomics to provide therapeutic benefits in hepatic inflammation[J].PeerJ,2023,11:e15829.
[58] 董学前.耐温超氧化物歧化酶产业化开发及其缓解霉菌毒素对动物毒性的研究[D].济南：山东大学，2022.DONG X Q.Industrial development of a thermo-tolerant superoxide dismutase and its functions of alleviating the mycotoxins toxicity to animals[D].Jinan:Shandong University,2022.(in Chinese)
[59] TANG M,FANG R,XUE J,et al.Effects of catalase on growth performance,antioxidant capacity,intestinal morphology,and microbial composition in Yellow broilers[J].Frontiers in Veterinary Science,2022,9:802051.
[60] SURAI P F,FISININ V I.Antioxidant-prooxidant balance in the intestine:Applications in chick placement and pig weaning[J].Journal of Veterinary Science & Medicine,2015,3(1):66-84.
[61] ROSSI L,DELL’ANNO M.Novel antioxidants for animal nutrition[J].Antioxidants,2024,13(4):438.
[62] LAMBETH J D.NOX enzymes and the biology of reactive oxygen[J].Nature Reviews Immunology,2004,4(3):181-189.
[63] LAVIN S R,KARASOV W H,IVES A R,et al.Morphometrics of the avian small intestine compared with that of nonflying mammals:A phylogenetic approach[J].Physiological and Biochemical Zoology,2008,81(5):526-550.
[64] MORGAN N K,WALK C L,BEDFORD M R,et al.The effect of dietary calcium inclusion on broiler gastrointestinal pH:Quantification and method optimization[J].Poultry Science,2014,93(2):354-363.
[65] 崔红霞.超氧化物歧化酶模拟物对肉仔鸡生长性能和抗氧化的影响[J].哈尔滨：东北农业大学,2019.CUI H X.Effects of superoxide dismutase mimic on performance and antioxidant of broilers[J] Harbin:Northeast Agricultural University,2019.(in Chinese)
[66] 王思博,崔红霞,杨季,等.超氧化物歧化酶模拟物对氧化应激IPEC-J2细胞抗氧化性能的影响[J].中国畜牧兽医,2021,48(5):1566-1573.WANG S B,CUI H X,YANG J,et al.Effect of superoxide dismutase mimetic on antioxidant capacity of oxidatively damaged IPEC-J2 cells[J].China Animal Husbandry & Veterinary Medicine,2021,48(5):1566-1573.(in Chinese)
[67] SIRISENA D,PERERA N C N,GODAHEWA G I,et al.A manganese superoxide dismutase (MnSOD) from red lip mullet,Liza haematocheila:Evaluation of molecular structure,immune response,and antioxidant function[J].Fish & Shellfish Immunology,2019,84:73-82.
[68] MEIMANDIPOUR A,SHUHAIMI M,HAIR-BEJO M,et al.In vitro fermentation of broiler cecal content:The role of Lactobacilli and pH value on the composition of microbiota and end products fermentation[J].Letters in Applied Microbiology,2009,49(4):415-420.

Effects of Dietary Superoxide Dismutase Supplementation on Growth Performance, Immune Performance,Antioxidant Capacity and Intestinal Function of Broilers

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Zhengfei, TANG Anxing, SUN Jindong, FU Lixiang, WANG Xingxian, ZHANG Shiyun, YANG Liangyu, NIU Guoyi, TAO Linli. Study on the Appropriate Dietary Crude Protein Level of Yanjin Black-bone Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1543-1553.
[2]	LAI Qiushuang, LI Lingshuo, LI Shuyi, TIAN Wei, YU Qingting, ZHANG Kanglin, LI Depeng, LIU Jun, JIANG Fei, PENG Zhongli, WU Jianping, HUANG Yanling. Effects of Rumen-protected Methionine Supplementation in Diets with Different Protein Levels on Growth Performance,Rumen Fermentation and Apparent Digestibility of Nutrients in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1567-1578.
[3]	HONG Jiajun, XIAO Jinhua, ZHANG Zheng, YANG Yuting, FANG Chun, HAN Xu. Effect of sod Gene Deletion on the Anti-oxidative Stress in Listeria monocytogenes 10403S [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1776-1783.
[4]	WANG Tongyi, CHEN Jing, JIANG Xinyu, ZHANG Yifei, WANG Hairong, HU Bing, YANG Xujin, YUN Xueyan, GAO Aiwu. Effects of Supplementary Complex Plant Additive on Antioxidant Capacity and Gel Properties of Post-slaughter Mutton [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1045-1056.
[5]	WEI Ying, DUAN Yehui, DENG Jinping. Research Progress on the Application of Flavor Amino Acids in Pig and Chicken Production [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1089-1101.
[6]	OU Jiancun, HUANG Jian, QIU Ting, CEN Mingzhu, ZHENG Chaojun, WANG Wenhe, HUANG Weilong, ZHANG Huihua. Effects of 7 Essential Amino Acids Balanced Low Protein Diet on Growth, Nutrient Digestion and Intestinal Tissue Morphology of Qingyuan Partridge Chickens at 31 to 60 Days of Age [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1102-1112.
[7]	FAN Qiuli, TAO Zhengguo, LI Hui, GOU Zhongyong, WANG Yibing, LIN Xiajing, YE Jinling, JIANG Shouqun. Effects of α-mangostin on Growth Performance,Immune Function, Antioxidant Capacity and Detoxification Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1123-1130.
[8]	HUANG Xuan, MA Yuyong, LIU Yang, LI Chuang, JIANG Guitao, ZHANG Xu, DENG Ping, WAN Weican, HE Xu, DAI Qiuzhong. Feeding Effects of Granular Alfalfa and Fresh Pasture Partially Replacing Complete Feed on Linwu Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1159-1165.
[9]	DONG Liyue, FAN Zuokun, TANG Meihui, TANG Jianqiang, YU Hailiang, ZHANG Tao, ZHANG Genxi, XIE Kaizhou, ZHAO Zhenhua, DAI Guojun. Effect of Eimeria tenella on Growth Performance,Immune Organs and Intestinal Morphology of Yellow-feathered Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1263-1271.
[10]	YANG Xinshuo, CHANG Danyi, XU Dandan, ZHANG Haihua, MA Qiugang, HUANG Shimeng. Advances in the Impact of Tryptophan and Its Bacterial Metabolites on Intestinal Barrier Function and Growth Performance in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 636-644.
[11]	ZHONG Ming, GENG Longjun, CUI Lianhua, XU Ziyan, YAN Changguo, FENG Jian, CAI Hongfan, ZHANG Xu. Effects of Curcumin on Growth Performance, Apparent Digestibility of Feed Nutrients and Blood Indexes of Yanbian Yellow Breeding Cattle Under Heat Stress [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 655-667.
[12]	LI Jing, DONG Ying. Effects of Bacillus subtilis on Growth Performance,Intestinal Morphology, Serum Biochemical and Immune Indexes in Nursery Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 678-685.
[13]	LIU Shixiong, LI Xiao, LI Xueqiang, XU Zixuan, LI Songjian, DU Rui, XUE Chen, LIU Dacheng. Effects of Compound Bacteria Culture on Growth Performance,Immune Function and Vaccine Antibody Titer of Mongolian-Han Crossbred Mutton Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 718-729.
[14]	FENG Yi, YANG Yunyun, LUO Yongrong, WU Peng, WANG Yan, YANG Ying, CHEN Jiangfeng, JIANG Haibo, WEN Ming. Effect of Dietary Addition of Rosa roxburghii Tratt. Residue on Intestinal Histomorphology,Antioxidant Capacity,Immunity and Metabolomics in Common Carp (Cyprinus carpio) [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 80-93.
[15]	WANG Jing, LIANG Guichen, FU Rui, WANG Zewu, ZANG Changjiang, LI Fengming. Effects of Dietary Crude Protein Level on Growth and Slaughtering Performance and Intestinal Microflora Structure of Squabs [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 107-120.