唾液乳杆菌无细胞上清液对金黄色葡萄球菌溶血活性的抑制作用研究

doi:10.16431/j.cnki.1671-7236.2025.04.040

Abstract

Abstract: 【Objective】 The objective of this experiment was to investigate the effect of cell-free culture supernatant (CFCS) of Lactobacillus salivarius (L.salivarius) on the hemolytic activity of Staphylococcus aureus (S.aureus)and to compare the effect of different culture conditions on the bacteriostatic effect of CFCS of L.salivarius.【Method】 The minimum inhibitory concentration (MIC) and subinhibitory concentration (SIC) of CFCS of L.salivarius on S.aureus were determined by broth dilution method.The influence of CFCS of L.salivarius on the hemolytic activity of S.aureus was detected by semi-quantitative method.The relative expression levels of α hemolysin (hla) and AraC/XylS family transcriptional regulators rbf gene in S.aureus were determined by Real-time quantitative PCR.The effects of CFCS of L.salivarius on mortality,organ bacteria load and histopathological changes of mice were detected by pathogenicity test.The effects of different medium and culture conditions on the antibacterial activity of CFCS of L.salivarius were determined by Oxford Cup double-layer agar diffusion method,respectively.【Result】 The MIC and SIC of CFCS of L.salivarius against S.aureus were 31.25 and 3.906 mg/mL,respectively.After treatment with CFCS of L.salivarius with MIC,compared with positive control group,the hemolytic activity of S.aureus was extremely significantly decreased (P＜0.01),the relative expression level of hla gene was significantly decreased (P ＜ 0.05),and the relative expression level of rbf gene was extremely significantly increased (P＜0.01).The results of pathogenicity test in mice showed that after treatment with MIC of CFCS of L.salivarius,the bacteria load in liver,kidney and spleen of mice infected with S.aureus was extremely significantly decreased (P＜0.01),and the lesions of liver,kidney and spleen tissues were significantly reduced.The fermentation of CFCS of L.salivarius in MRS medium had the best inhibitory effect on S.aureus,and the inhibitory zone diameter was 17.64 mm,followed by TPY medium (12.83 mm).Based on MRS medium,when the initial glucose content was 4%,the initial nitrogen source content was 1.1%,the initial pH was 4.0 and the fermentation time was 48 h,CFCS of L.salivarius had the best bacteriostatic effect on S.aureus.【Conclusion】 CFCS of L.salivarius could inhibit the hemolytic activity of S.aureus and reduce its pathogenicity.The change of culture conditions could significantly affect the antibacterial activity of CFCS of L.salivarius.

Key words: Lactobacillus salivarious; cell-free culture supernatant (CFCS); Staphylococcus aureus; hemolytic activity; pathogenicity

CLC Number:

S831.5

XING Guofeng, DANG Yifan, ZHAO Yao, SU Fanfan, WU Zihao, LI Tao. Inhibition of CFCS of Lactobacillus salivarius on Hemolytic Activity of Staphylococcus aureus[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1884-1894.

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Inhibition of CFCS of Lactobacillus salivarius on Hemolytic Activity of Staphylococcus aureus

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