新疆南疆部分地区绵羊体表寄生软蜱种类鉴定及其携带绵羊无浆体的检测分析

doi:10.16431/j.cnki.1671-7236.2025.04.034

Abstract

Abstract: 【Objective】 This study was aimed to investigate the species of parasitic argasid ticks on the surface of sheep and the infection status of Anaplasma ovis in certain areas of Southern Xinjiang,China,and provide a scientific basis for the prevention and control of Anaplasma ovis disease in the region.【Method】 A total of 134 samples of hungry argasid ticks from sheep were collected from Yutian,Yingjisha,Jiashi,Qira,Wushi and Awat in Xinjiang from 2022 to 2023.These samples were identified through morphological and molecular biology methods.PCR technique was employed to amplify the 16S rDNA gene of argasid ticks and the major surface protein 4 (MSP4) gene of Anaplasma ovis.Furthermore,sequences with high similarity were retrieved from GenBank for local multiple sequence alignments,and phylogenetic tree was constructed using Mega X.【Result】 After morphological identification,the dorsum of the argasid ticks lacked a shield and featured a scattered distribution of stellate fossae,which were initially identified as Ornithodoros. Through molecular biological identification,its 16S rDNA gene sequence was found to be closest to Ornithodoros lahorensis in Xinjiang strain (accession No.：ON159483) in GenBank,with 100% similarity.The detection of Anaplasma ovis in argasid ticks by PCR had a positive rate of 14.92% (20/134).The positive sequence was compared with the MSP4 gene sequence of Xinjiang (accession No.：OP503167) and Sudan (accession No.：KU497709) strains,and 6 base differences and 6 genotypes of Anaplasma ovis were identified,named as LA1,LA2,LA3,LY1,LY2 and LT1，respectively,the accession No.were as follows:PP997635-PP997640.Genetic evolutionary analysis revealed that the MSP4 gene sequence of Anaplasma ovis was closest to Sudan (accession No.：MF740812) and Pakistan (accession No.：MT311203) strains,with similarities ranging from 98.77% to 99.69%.【Conclusion】 The argasid ticks in Southern Xinjiang were all identified as Ornithodoros lahorensis,and the positive rate of Anaplasma ovis from sheep in these samples was 14.92%.The genotype of Anaplasma ovis from sheep in Southern Xinjiang were predominantly type LT1.The results had enriched the Anaplasma ovis database of Xinjiang，and provided a theoretical reference for the epidemiological study of pathogens carried by argasid ticks,as well as for the prevention and control of tick-borne diseases in the region.

Key words: argasid ticks; Ornithodoros lahorensis; Anaplasma ovis; MSP4 gene; genetic evolutionary

CLC Number:

S852.74⁺6

ZHU Huiru, ZHAO Yudong, LIU Dandan, JIANG Bingbing, ZHANG Zetian, MA Sinan, LI Longtao, HUANG Jingrenjie, WANG Yongqing, Bayinchahan, ZHANG Yang, LI Yongchang, ZHANG Wei. Species Identification of Parasitic Argasid Ticks and Detection of Anaplasma ovis from Sheep in Some Areas of Southern Xinjiang,China[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1825-1835.

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Species Identification of Parasitic Argasid Ticks and Detection of Anaplasma ovis from Sheep in Some Areas of Southern Xinjiang,China

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