内蒙古地区牛呼吸道感染的主要病原菌及支原体分离鉴定及耐药性和毒力基因检测

doi:10.16431/j.cnki.1671-7236.2025.03.034

Abstract

Abstract: 【Objective】 This study was aimed to understand the infection,drug resistance and virulence genes of the main causative organisms and Mycoplasma of bovine respiratory diseases in a large-scale ranch in Inner Mongolia,and provide medication basis for the prevention and treatment of bovine respiratory diseases in this ranch.【Method】 The lung tissues of dead cattle were collected for isolation and culture of pathogenic bacteria,biochemical identification,16S rRNA gene sequencing,PCR identification,drug susceptibility test,drug resistance genes and virulence genes detection.【Result】 One strain of Pasteurella multocida type A and one strain of Mannheimia haemolytica type A6 were isolated from the TSA plate,which showed rounded,grayish-white,transparent or translucent colonies,and the amplification of specific and serotypic genes was in accordance with the expected bands.One strain of Mycoplasma bovis was isolated from the solid medium of PPLO,which appeared in the solid medium of PPLO,and showed the appearance of omelette in the microscopic examination,and the amplification of specific genes was in accordance with the expected bands.The 16S rRNA sequencing and aligning results showed that the three pathogens had similarities of over 99% with Pasteurella multocida,Mannheimia haemolytica and Mycoplasma bovis,respectively.The results of drug resistance analysis and virulence gene detection showed that Pasteurella multocida and Mannheimia haemolytica were highly sensitive to oxfloxacin,and sensitive to kanamycin,amikacin,gentamicin and other antimicrobials.Pasteurella multocida carried resistance genes strA,strB,tetA,tetB,qnrA and bla_ROB,and 22 virulence genes such as exbB,exbD,ompA and psl.Mannheimia haemolytica carried resistance genes strA and strB,and four virulence genes such as gcp,gs60,lktC and tbpB.Mycoplasma bovis was resistant to streptomycin,erythromycin,clindamycin,intermediary to ciprofloxacin,amikacin and kanamycin,and sensitive to gentamicin,ofloxacin,enrofloxacin and tetracycline,carrying resistance genes strA,strB,mph and msr-E,and the virulence genes of VspX,VspY2,VpmA and P81.【Conclusion】 The respiratory disease of sick and dead cattle in this ranch was caused by Pasteurella multocida,Mannheimia haemolytica and Mycoplasma bovis,and the isolated strains carried a variety of virulence genes,it was recommended to use oxfloxacin as the main therapeutic antimicrobial drug for the treatment of bovine respiratory disease in the clinic and the use of antimicrobial drugs such as erythromycin and clindamycin were not recommended.

Key words: Pasteurella multocida; Mannheimia haemolytica; Mycoplasma bovis; separation and identification; resistance; virulence genes

CLC Number:

YIN Kaiwen, MAO Wei, CAO Jinshan, SUN Yue, DONG Haiyan, HAN Kaifan, WANG Bo, LI Peifeng, ZHANG Zhidan, FAN Hongliang, GUO Yu, ZHAO Hongxia. Isolation and Identification of Major Pathogenic Bacteria and Mycoplasma Causing Bovine Respiratory Tract Infection and Detection of Drug Resistance and Virulence Genes[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1328-1341.

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Isolation and Identification of Major Pathogenic Bacteria and Mycoplasma Causing Bovine Respiratory Tract Infection and Detection of Drug Resistance and Virulence Genes

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