保定地区猫泛白细胞减少症病毒VP2基因的遗传进化分析

doi:10.16431/j.cnki.1671-7236.2025.02.032

Abstract

Abstract: 【Objective】 The objective of this study was to investigate the prevalence of Feline panleukopenia virus (FPV) and the genetic variation of FPV VP2 gene in Baoding. 【Method】 The feces and nasal secretions samples of 20 cats suspected to be infected with feline panleukopenia (FP) were randomly collected from several pet hospitals in Baoding,and the FPV VP2 whole gene sequence was obtained by PCR amplification and sequencing.The phylogenetic tree was constructed by Mega 7.0 software,and the nucleotide sequence similarity and key amino mutation sites were analyzed by MegAlign software. 【Result】 16 FPV VP2 specific bands with length of 1 755 bp were amplified from the fecal samples of 20 cats,and the sequence analysis showed that the full length of FPV VP2 gene was 1 755 bp.The isolated FPV belonged to G1 subtype,which was closely related to Japanese strain V211 (accession No.:AB054227) and far related to FPV standard strain CU-4 (accession No.:M38246).The nucleotide sequence similarity among 16 FPV VP2 genes was 99.1%-100%.13 key amino acid sites of 16 VP2 protein sequences were the same.Compared with FPV standard strain CU-4,there was an I-T mutation at amino acid 101.Compared with the key amino acid sites of 5 reference strains CPV VP2,only the 101 amino acid site was the same,and other sites had different degrees of variation. 【Conclusion】 G1 FPV was predominant in Baoding area,and the VP2 gene had strong genetic stability.The results enriched the epidemiological data of FPV and provide a theoretical basis for the prevention of FPV in Baoding area.

Key words: Feline leukopenia virus (FPV); VP2 gene; genetic evolution; amino acid site

CLC Number:

S852.65⁺9.2

ZOU Chang, HU Yue, LI Haihua, QIAO Jiayun. Genetic Evolution Analysis of VP2 Gene of Feline Panleukopenia Virus in Baoding Area[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 823-831.

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Genetic Evolution Analysis of VP2 Gene of Feline Panleukopenia Virus in Baoding Area

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