槲皮素对沙门菌生物被膜形成的抑制作用研究

doi:10.16431/j.cnki.1671-7236.2024.11.039

Abstract

Abstract: 【Objective】 The objective of this experiment was to study the inhibitory effect of quercetin on the biofilm formation of Salmonella and its mechanism. 【Method】 The growth curve of Salmonella biofilms was plotted by crystal violet staining,and the minimum biofilm inhibitory concentration (MBIC) of quercetin on Salmonella was determined.The bacteria were treated with quercetin,and the metabolic activity and morphology of the bacteria in Salmonella biofilms were observed by XTT method and scanning electron microscope.The contents of exopoly saccharides,protein and DNA of Salmonella biofilm were detected by phenol-sulfuric acid method,BCA method and ultraviolet spectrophotometer，respectively.The hydrophobicity and motility of Salmonella were determined by bacteria adhesion to hydrocarbons and soft agar method. 【Result】 The results of growth curve showed that the adhesion,aggregation,maturation and dispersion periods of Salmonella biofilms were 0-48,48-72,72-120 and 120-168 h,respectively.The MBIC of quercetin against Salmonella biofilms was 256 μg/mL.The metabolic activity of bacteria in Salmonella biofilm treated with quercetin was extremely significantly decreased at different times (P＜0.01).Scanning electron microscopy observation showed that after quercetin treatment,the structure of Salmonella biofilms was destroyed and the membrane-like substance disappeared.Compared with control group,the exopoly saccharides,protein and DNA contents of Salmonella biofilms in quercetin group were extremely significantly decreased (P＜0.01).The hydrophobicity index,swimming motility diameter and swarming motility diameter of Salmonella decreased extremely significantly (P＜0.01). 【Conclusion】 Quercetin inhibited biofilm formation by affecting the metabolic activity of bacteria in Salmonella,reducing the production of extracellular polymeric substances,and interfering with surface hydrophobicity and motility of Salmonella.

Key words: quercetin; Salmonella; biofilm

CLC Number:

S816.7

WANG Qiaoyu, ZHEN Mingzhe, ZHANG Nianjie, YAN Dongxue, WANG Xin. Inhibitory Effect of Quercetin on Biofilm Formation of Salmonella[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 5043-5050.

References

[1] ZHANG M,HAN W,GU J,et al.Recent advances on the regulation of bacterial biofilm formation by herbal medicines[J].Frontiers in Microbiology,2022,13:1039297.
[2] GLOAG E S,FABBRI S,WOZNIAK D J,et al.Biofilm mechanics:Implications in infection and survival[J].Biofilm,2020,2:100017.
[3] 吴同垒,冀梦瑶,周诗淼,等.GlpK影响肠炎沙门菌生物被膜形成[J].中国兽医学报,2022,42(5):920-925.
WU T L,JI M Y,ZHOU S M,et al.Glpk affects the biofilm formation of Salmonella Enteritis[J].Chinese Journal of Veterinary Science,2022,42(5):920-925.(in Chinese)
[4] ZHAO A,SUN J,LIU Y.Understanding bacterial biofilms:From definition to treatment strategies[J].Frontiers in Cellular and Infection Microbiology,2023,13:1137947.
[5] ZENG Y,NIKITKOVA A,ABDELSALAM H,et al.Activity of quercetin and kaemferol against Streptococcus mutans biofilm[J].Archives of Oral Biology,2019,98:9-16.
[6] 崔乃菠.基于生物膜模型研究中药的体外抗感染效应[D].天津:天津中医药大学,2022.
CUI N B.Research of anti-infection of traditional Chinese medicine based on biofilm[D].Tianjin:Tianjin University of Traditional Chinese Medicine,2022.(in Chinese)
[7] YU J,WANG F,SHEN Y,et al.Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms[J].BMC Oral Health, 2022,22(1):350.
[8] 张慧,郭晓梅,白婧,等.3,3'-二吲哚甲烷对肠炎沙门氏菌生物被膜的抑制作用[J].中国测试,2022,48(1):60-67.
ZHANG H,GUO X M,BAI J,et al.Antibiofilm activity of 3,3'-diindolomethane on Salmonella Enteritidis [J].China Measurement & Test,2022,48(1):60-67.(in Chinese)
[9] LI X,GUAN C,HE Y,et al.Effects of total alkaloids of Sophora alopecuroides on biofilm formation in Staphylococcus epidermidis[J].BioMed Research International, 2016,2016:4020715.
[10] SHE P,WANG Y,LIU Y,et al.Effects of exogenous glucose on Pseudomonas aeruginosa biofilm formation and antibiotic resistance[J].Microbiology Open,2019,8(12):e933.
[11] 殷川平.异绵马素BB对表皮葡萄球菌生物被膜形成的作用及其机制研究[D].广州:广东药科大学,2021.
YIN C P.Study on the effect and mechanism of isoasipidin BB on the biofilm formation of Staphylococcus epidermidis[D].Guangzhou:Guangdong Pharmaceutical University,2021.(in Chinese)
[12] 郭航帆,王萍,王颖.龙葵提取物对大肠杆菌、粪肠球菌及其生物膜形成的抑制作用[J].中国农业科技导报,2023,25 (10):152-164.
GUO H F,WANG P,WANG Y.Inhibitory effect of Solanum nigrum L extracts on Escherichia coli and Enterococcus faecalis and biofilm formation[J].Journal of Agricultural Science and Technology,2023,25 (10):152-164.(in Chinese)
[13] 宋雪艳,张鲁星,谢翠萍,等.cpxR基因对大肠杆菌E41株生物学特性及生物被膜形成的影响研究[J].中国预防兽医学报,2023,45 (5):466-472.
SONG X Y,ZHANG L X,XIE C P,et al.Effects of cpxR gene on biological characteristics and biofilm formation of Escherichia coli E41[J].Chinese Journal of Preventive Veterinary Medicine,2023,45(5):466-472.(in Chinese)
[14] 张雨.茄镰刀菌复合体生物被膜的形成过程及1,8-桉叶油素对其的影响研究[D].成都:四川农业大学,2023.
ZHANG Y.Study on the formation process of biofilm of Fusarium solani species complex and the effect of 1,8-cineole on it [D].Chengdu:Sichuan Agricultural University,2023.(in Chinese)
[15] 田淼梅,李昕,郭思维,等.抗细菌生物被膜研究新进展[J].中国感染与化疗杂志,2024,24(1):108-112.
TIAN M M,LI X,GUO S W,et al.Research progress in addressing bacterial biofilms[J].Chinese Journal of Infection and Chemotherapy,2024,24(1):108-112.(in Chinese)
[16] 张君怡,王静怡,巴巨伟,等.细菌生物被膜检测与清除方法研究进展[J].食品研究与开发,2020,41(20):218-224.
ZHANG J Y,WANG J Y,BA J W,et al.Development on detection and removal of bacterial biofilm[J].Food Research and Development, 2020,41(20):218-224.(in Chinese)
[17] 刘宝宝,汪洋,易力,等.细菌生物被膜检测与分析方法[J].微生物学通报,2018,45(10):2263-2270.
LIU B B,WANG Y,YI L,et al.Detection and analysis methods of bacterial biofilm[J].Microbiology China,2018,45(10):2263-2270.(in Chinese)
[18] ALONSO B，CRUCES R,PEREZ A,et al.Comparison of the XTT and resazurin assays for quantification of the metabolic activity of Staphylococcus aureus biofilm[J].Journal of Microbiological Methods, 2017,139:135-137.
[19] 张欣,潘晨浩,赵瑞利,等.抗菌肽Temprine-La(FS)抑制2型猪链球菌生物被膜形成的研究[J].中国畜牧兽医,2021,48(1):324-337.
ZHANG X,PAN C H,ZHAO R L,et al.Inhibitory effect of antimicrobial peptide Temprine-La(FS) on biofilm formation of Streptococcus suis type 2[J].China Animal Husbandry & Veterinary Medicine,2021,48(1):324-337.(in Chinese)
[20] 康馨匀.槲皮素对金黄色葡萄球菌生物被膜抑制作用机制的研究[D].银川:宁夏大学,2023.
KANG X Y.Study on the inhibition mechanism of quercetin on Staphylococcus aureus biofilm [D].Yinchuan:Ningxia University,2023.(in Chinese)
[21] 胡锦松,陈豪泰,张杰,等.细菌生物被膜鉴定方法的研究进展[J].中国兽医科学,2010,40(11):1194-1199.
HU J S,CHEN H T,ZHANG J,et al.Advances in the common identification methods of bacterial biofilm[J].Chinese Veterinary Science,2010,40(11):1194-1199.(in Chinese)
[22] 余生玲,申光辉,黄廷,等.藤椒精油对腐败解淀粉芽孢杆菌DY1a生物被膜的抑制作用[J].微生物学通报,2022,49 (6):2135-2151.
YU S L,SHEN G H,HUANG T,et al.Antibiofilm effect of Zanthoxylum armatum DC.essential oil against spoilage Bacillus amyloliquefaciens DY1a[J].Microbiology China,2022,49 (6):2135-2151.(in Chinese)
[23] 刘静聪,方金玉,朱军莉.细菌生物被膜基质的研究进展[J].微生物学报,2022,62(1):47-56.
LIU J C,FANG J Y,ZHU J L.A brief review of biofilm matrix in structured microbial communities[J].Acta Microbiologica Sinica,2022,62(1):47-56.(in Chinese)
[24] 卢惠芳,黄健,闵迅.细菌的鞭毛运动及其调控机制研究进展[J].医学综述,2022,28(14):2711-2716.
LU H F,HUANG J,MIN X.Research progress of bacterial flagellar motility and its regulatory mechanism[J].Medical Recapitulate,2022,28(14):2711-2716.(in Chinese)
[25] ROY P K,PARK S H,SONG M G,et al.Antimicrobial efficacy of quercetin against Vibrio parahaemolyticus biofilm on food surfaces and downregulation of virulence genes[J].Polymers, 2022,14(18):3847.

Inhibitory Effect of Quercetin on Biofilm Formation of Salmonella

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