Abstract: The purpose of the experiment was to screen the effective ingredients of Wujin granules in the treatment of lamb dysentery through the mining function of network pharmacology,and explain its potential targets and possible pathways.According to the Traditional Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP),the main effective compounds of each single medicine of Wujin granules were analyzed,and at the same time their corresponding targets were searched.Compounds without targets were analyzed again by the Swiss Target Prediction database for their targets,it was predicted that the compound with factual target and its corresponding target protein were finally obtained.Cytoscape 3.7.1 software was used to construct the network diagram of the effective ingredient-target protein,the selected target protein was uploaded to the String online database to construct Wujin granules acting on the protein-protein interaction (PPI) map of lamb (Ovis aries) dysentery,and finally uploaded the core targets in the PPI network to the DAVID database for GO function and KEGG pathway enrichment analysis,and at the same time performed gene function cluster analysis.The results showed that a total of 22 compounds with de facto targets were screened,including kaempferol,stigmasterol,methyl arachidonic acid,quercetin,ellagic acid,and so on,and a total of 129 compounds corresponding to target proteins were deduplicated.The PPI network diagram showed that the number of nodes was 74,the number of edges was 287,the average node degree was 7.76,and a total of 67 core target proteins,including INSR,NR3C1,ESR1,PLG,PGR,and so on.GO function enrichment analysis showed that 54 items were related to biological process (BP) (FDR<0.05),11 items were related to cell component (CC) (FDR<0.05),22 items were related to molecular function (MF) (FDR<0.05).GO function enrichment gene items with more count included transcription,plasma membrane,zinc ion binding,and so on,KEGG pathway enrichment results involved 50 genes,and 43 regulatory pathways involved in gene coordination,neuroactive ligand-receptor interaction,pathways in cancer,complement and coagulation cascades,and so on,involved more genes.Gene function classification results showed that of 64 cores among the target genes,12 genes had clustering results.They were divided into two groups with different functions,namely group 1 (enrichment score 6.11,involving 7 core target genes) and group 2 (enrichment score 2.84,involving 5 core target genes).In conclusion,Wujin granules might act on a variety of different targets through a variety of compounds,and participate in the regulation of multiple pathways to achieve the therapeutic mechanism of lamb dysentery.

Key words: network pharmacology; Wujin granules; lamb dysentery; target; mechanism of action