不同亚油酸水平培养基对溶纤维丁酸弧菌脂肪酸合成的影响

doi:10.16431/j.cnki.1671-7236.2018.12.011

Abstract

Abstract:

To explore the effect of linoleic acid on the synthesis of fatty acids of Butyrivibrio fibrisolvens in ruminant animals,different levels of linoleic acid medium were used to conduct an anaerobic culture test with Butyrivibrio fibrisolvens.Linoleic acid was added to the culture medium to make its concentrations in test groups 1 (control group),2,3 and 4 were 0,2.5,5.0 and 7.5 mg/100 mL when D_{600 nm} value of bacteria reached 1.0.The bacteria fluid was collected and tested for 35 kinds of fatty acids after cultured for 24 h.The results showed that there were no significant difference in the contents of C8:0,C11:0,C14:0,C15:0,C17:0,C17:1,C18:1(n-9)C,C18:2(n-6)C,C18:3(n-6),C20:0,C20:2,C20:3(n-3),C20:3(n-6),C20:5(n-3),C21:0,C22:0,C22:2,C22:1(n-9)and C22:6(n-3) with the increase of linoleic acid (P>0.05);The amount of C10:0,C12:0,C16:0,C18:0,C20:4(n-6),C23:0 and C24:1 were significantly decreased response to the linoleic acid addition (P<0.05).The contents of C15:1 and C18:3(n-3) were significantly increased in 2.5 mg/100 mL linoleic acid group (P<0.05),the amount of C14:1 in control group was the highest and that would increase with the linoleic acid increase.The amount of C24:0 in 7.5 mg/100 mL linoleic acid group was the highest.In conclusion,linoleic acid had a certain effect on the synthesis of fatty acids of Butyrivibrio fibrisolvens,it could inhibit the synthesis of C10:0,C12:0,C16:0,C18:0,C23:0,C14:1 and C20:4(n-6),increase the synthesis of C15:1,C16:1 and C18:3(n-3) which would decrease when the linoleic acid content exceed a certain value.

Key words: Butyrivibrio fibrisolvens; linoleic acid; culture in vitro; fatty acid synthesis

CLC Number:

Q936

WANG Fu, CHEN Shengchang, HAN Yong, WU Xian, WANG Yinchen, ZHANG Kaikai, WANG Qingfeng, YANG Linhua, LU Xiaoluo, ZHANG Taotao. Effects of Different Linoleic Acid Contents in Medum on the Synthesis of Fatty Acids of Butyrivibrio fibrisolvens[J]. , 2018, 45(12): 3395-3401.

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Effects of Different Linoleic Acid Contents in Medum on the Synthesis of Fatty Acids of Butyrivibrio fibrisolvens

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