蓝狐MITF-M基因序列扩增及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.01.006

Abstract

Abstract:

In this study,to investigate the core promoter activity region, transcription factor binding site,the structure and function of encoding protein and the expression and regulation mechanism of Blue fox MITF-M gene,the skin tissue samples (1 cm×1 cm) was collected from adult Blue fox,the partial sequence of MITF-M gene was obtained by RT-PCR and sequencing technique for the first time. The length of the Blue fox MITF-M gene was 3 880 bp (including 2 262 bp 5' flanking region,1 260 bp CDS region and 358 bp 3' flanking region), and encoding 419 amino acid residues. The bioinformatics analysis showed that there were potential promoter regions at -86 to -336 bp in the 5' flanking region of MITF-M gene, and some regulatory elements (TATA box,CAAT box,GC box and CRE) and transcription factors (CREB,LSF and Sp1) were found. The protein encoded by MITF-M gene was unstable and hydrophilic which mainly located in nucleus and cytoplasm. Subcellular localization results showed that the protein had a higher probability in nucleus (65.2%),cytoplasm (17.4%)and mitochondrion (8.7%),the results of secondary structure prediction by DNAMAN software found that the protein was consist of α helix (33.66%),β sheet (16.66%) and random coil (49.68%),and there was no transmembrane region and signal peptide. Multiple sequence alignment and phylogenetic tree analysis showed that Blue fox had the closet relationship with dog and a high homology with mammal and poultry (>89.1%) indicated that the MITF-M gene coding region was conservative in the course of evolution. This study laid the theoretical foundation for the in-depth study of the molecular genetic mechanism of the MITF-M gene regulating the hair color of the fox.

Key words: Blue fox; MITF-M gene; 5'flanking region; coding region; bioinformatics analysis

CLC Number:

Q786

GUO Min, ZHANG Donglin, LIU Yanjun, PENG Yongdong, WANG Jiantao, FU Zhixin, DONG Shuzhen, LIU Zhengzhu, GONG Yuanfang, LI Xianglong. Sequence Amplification and Bioinformatics Analysis of MITF-M Gene in Blue Fox (Alopex lagopus)[J]. , 2018, 45(1): 47-56.

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Sequence Amplification and Bioinformatics Analysis of MITF-M Gene in Blue Fox (Alopex lagopus)

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