猪SKP1基因的克隆及其在梅山猪与杜洛克猪卵泡中的表达研究

doi:10.16431/j.cnki.1671-7236.2015.07.003

Abstract

Abstract: To detect the expression pattern of S-phase kinase association protein 1 (SKP1) gene in during porcine follicle development.The coding sequence (CDS) of SKP1 gene was cloned from porcine follicular tissue.To analyze the tissue expression profiling of SKP1 gene and its expression level in follicles with different diameter (S,M1,M2,L) from Duroc and Meishan pigs were investigated by Real-time fluorescence quantitative PCR.The results showed that the length of CDS of porcine SKP1 was 492 bp,and it's similarity with that of Ovis aries,Homo sapiens,Pan troglodytes,Bos taurus and Rattus were 93.10%,92.90%,92.29%,91.89% and 89.86%,respectively.SKP1 mRNA was expressed in all tested tissues (muscle,fat,heart,liver,spleen,lung,kidney,stomach,duodenum,ovarian,tubal,uterine,uterine horn,pituitary,corpus luteum,brain,hypothalamus),and especially high in uterine,spleen and tubal.Both in Meishan pig.The expression of SKP1 mRNA in S,M1 and M2 follicles were higher than Duroc pig.Especially,the expression in Meishan pig M1 and M2 follicles were significantly higher than Duroc pig (P<0.01),respectively 2.39,2.82 times,and the expression of L follicle in Duroc pig was higher than Meishan pig,which suggested that SKP1 gene might be involved in the process of procine's follicular development.

Key words: porcine; SKP1 gene; follicle; expression

CLC Number:

XU Meng-si, HUANG Tao, LIU Li-juan, YANG Fei, LU Hui-wen, ZHAI Teng-jiao, CHEN Ya-nan. Cloning of Porcine SKP1 Gene and it's Expression Studies between Meishan and Duroc Pigs with Follicle[J]. , 2015, 42(7): 1640-1646.

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Cloning of Porcine SKP1 Gene and it's Expression Studies between Meishan and Duroc Pigs with Follicle

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