伪狂犬病病毒gB蛋白抗原表位基因串联构建及原核表达

doi:10.16431/j.cnki.1671-7236.2015.04.006

Abstract

Abstract: The two dominant epitope sequences of pseudorabies virus (PRV) gB gene were joined together by overlap-extension PCR method with two pairs of primers. The products were inserted into pMD18-T vector. After properly sequenced, the multi-epitope sequence was linked with pET-32a(+) vector, and then transformed into BL21(DE3). By being induced with IPTG, the targeted protein was detected by SDS-PAGE and Western blotting. The results showed the fusion protein was successfully expressed with the size of 54 ku including 34 ku tandem protein and could be combined with monoclonal antibody against gB which showed that the expressed protein had good antigenicity.

Key words: pseudorabies virus (PRV); gB gene; epitope; tandem gene expression

CLC Number:

Q786

WANG Yu, JI Yi-kuan, CHENG Yi, XIANG Ke-yu, ZHANG Bao-shi, JU Chun-mei. Construction of Tandem Epitopes of gB Gene of Pseudorabies Virus and its Prokaryotic Expression[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(4): 810-815.

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Construction of Tandem Epitopes of gB Gene of Pseudorabies Virus and its Prokaryotic Expression

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