ASCL2基因在体细胞克隆牛和自然繁殖牛肺脏中的甲基化分析

Abstract

Abstract: In an effort to determine the reprogramming of ASCL2 gene in SCNT bovines，the bisulfite sequencing method （BSP） was used to analyze the DNA methylation status in promoter region of ASCL2 gene in lung tissues of SCNT cattle and natural breeding cattle.The results showed that the methylation level of ASCL2 promoter exhibited seriously hypermethylated in SCNT bovines （28.41%） compared with naturally breed bovines （7.62%）（P＜0.05）. Our results suggested that the abnormal DNA methylation of ASCL2 might be associated with the defects of lung organs in died SCNT bovines，which was one of the main reasons for neonatal death of SCNT animals.

Key words: ASCL2; reprogramming; SCNT bovine; BSP; DNA methylation; organ defect

WANG Meng-nan，ZHANG Ming-yue，LI Ning，LI Shi-jie. DNA Methylation Status of ASCL2 Gene in Lungs of SCNT Cattle and Naturally Reproduced Cattle[J]. .

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DNA Methylation Status of ASCL2 Gene in Lungs of SCNT Cattle and Naturally Reproduced Cattle

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