2株高致病性猪繁殖与呼吸综合征病毒分子特征分析

Abstract

Abstract: In order to find out the graph variation characteristics of porcine reproductive and respiratory syndrome virus (PRRSV) of Qianxinanin Guizhou province, the Nsp2 and GP5 genes of PRRSV isolated strain GZLPS and GZSB were cloned, sequenced and analyzed. The results showed that, the Nsp2 gene of 2 isolates contained 90 nucleotides for discontinuous deletion and 36 nucleotides for mutation. Furthermore, there were different replacements of nucleotides in GP5 gene. Not only Nsp2 gene, but also GP5 gene, the nucleotide homologies were above 96% and the amino acid homologies were above 95% with strain JXA1 and isolates in Guzizhou since 2011, but there were differencex in molecular variation among these strains. It indicated that the PRRSV had already behaved diverse on variation style of Qianxinan in Guizhou province.

Key words: highly pathogenic porcine reproductive and respiratory syndrome virus; molecular characteristics; analysis; Nsp2; GP5

LUO Cheng-bo, CUI Min, KONG Ling-ping, CHENG Zhen-tao, ZHOU Bi-jun, MAO Li-hong. Molecular Characteristics Analysis of Two Strains of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus[J]. China Animal Husbandry & Veterinary Medicine.

References

1 尹彦涛，夏平安，崔保安，等. 猪繁殖与呼吸综合征病毒河南分离株ORF3-7基因克隆与序列分析［J］. 中国兽医学报，2008，28(5)：496～500.
2 王仕荣，向晖，吴健敏，等. 广西“猪高热综合征”主要病原调查及PRRSV Nsp2基因变异分析［J］. 中国兽医学报，2009，29(3)：249～253.
3 刘飞，王开功，周碧君，等. 贵州省PRRSV分离株NSP2基因的克隆及序列分析［J］. 中国预防兽医学报，2010，32(12)：925～928.
4 成岩，着梦娇，王美君，等. 山东省规模化猪场PRRSV Nsp2变异株的感染情况调查［J］. 中国预防兽医学报，2013，35(7)：535～538.
5 冷雪，李真光，王凤雪，等. 高致病性猪繁殖与呼吸障碍综合征病毒NM1全基因测序及致病性分析［J］. 中国兽医学报，2012，32(12)：1752～1757.
6 张青娴，王克领，张立宪，等. 高致病性蓝耳病的研究进展［J］. 中国畜牧兽医，2008，35(9)：105～107.
7 杨小蓉，荫硕焱，潘梦，等. 一株高致病性猪繁殖与呼吸综合征病毒变异毒株的基因组特征［J］. 畜牧兽医学报，2012，43(2)：263～269.
8 胡鸿惠，曹振，赵铁柱，等. 高致病猪繁殖与呼吸综合征病毒JXA1 株Nsp2基因分段克隆表达及其单克隆抗体的制备［J］. 中国畜牧兽医，2009，36(10)：39～44.
9 郭宝清，陈章水，刘文兴. 从疑似PRRSV感染的流产胎儿分离PRRSV的研究［J］. 中国预防兽医学报，1996，22(4)：259～262.
10 高志强，郭鑫，杨汉春，等. 猪繁殖与呼吸综合征病毒缺失变异株的基因组特征［J］. 畜牧兽医学报，2005，36(6)：578～584.
11 扈鸿霞，张涵凇，赵灵燕，等. 2004—2010年浙江省及其周边地区PRRSV GP5基因的分子进化与变异分析［J］. 中国兽医科学，2011，41(11)：1124～1130.
12 Cho J G, Dee S A. Porcine reproductive and respiratory syndrome virus［J］. Theriogenology, 2006, 66(3): 655～662.
13 Dea S, Gagnon C A, Mardassi H, et al. Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: Comparison of the North American and European isolates［J］. Archives of Virology, 2000, 145(4): 659～688.
14 Li Y F, Wang X L, Jiang P, et al.Genetic variation analysis of porcine reproductive and respiratory syndrome virus isolated in China from 2002 to 2007 based on ORF5［J］.Vet Microbiology, 2009, 138(1～2): 150～155.
15 Pringle C R.Virus taxonomy 1996-A bulletin from theⅩth intemational congress of virology in jerusalem［J］.Archives of Virology, 1996, 141(71): 2251～2256.
16 Tian K G, Yu X L, Zhao T Z, et al. Emergence of fatal PRRSV variants: Unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark［J］. PLoS One, 2007, 2: e526.
17 Wang C, Lee F, Huang T S, et al.Genetic variation in open reading frame 5 gene of porcine reproductive and respiratory syndrome virus in Taiwan［J］.Veterinary Microbiology, 2008, 131(3～4): 339～347.
18 Yuan S S, Mickelson D, Murtaugh M P, et al. Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains［J］. Virus Research, 2001, 74 (189)： 99～110.
19 Zhou Y J, Yu H, Tian Z J, et al.Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008［J］.Virus Research, 2009, 144(1～2): 136～144.

[1]	LI Tianxiu, LI Xinpeng, DONG Xinxing, LAN Guoxiang, YAN Dawei, ZHU Jiawei. Amplification,Sequence Analysis and Tissue Expression Study of HMOX2 Gene in Lijiang Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2219-2231.
[2]	ZHAO Zhonglong, ZHANG Yong, YANG Hong, YANG Runqian, AI Zhaobi, YE Li, SHU Chang. Analysis of PPP3CA Gene Polymorphism and Its Association with Body Size Traits in Zhijin White Geese [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1729-1738.
[3]	LIN Weishan, MA Doudou, LEI Mengtong, WEI Bin, LI Guocai, WANG Guanghua, WANG Geping, JIAN Yingna, LI Xiuping. Epidemiological Investigation and Genetic Evolution Analysis of 5'-UTR of Bovine Viral Diarrhea Virus in Yaks in Haibei，Qinghai Province [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1241-1249.
[4]	LUO Qin, LIU Dingyu, LIU Baoling, QIAO Changhong, CHEN Xiangyu, HE Zhenwen, WANG Xiaohu, CHEN Jing, ZHANG Pian, HUANG Yuan, WANG Gang, LIU Hao, CAI Rujian. Research Progress on Antiviral Effect of Traditional Chinese Medicine Analyzed from Different Omics Perspectives [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 554-561.
[5]	YANG Jinlin, LI Hui, ZHAO Depeng, SHI Yushi, LONG Xia, TAN Qisong. Polymorphisms of EDNRB2 Gene and Its Association with Skin Color Traits in Chishui Black-bone Chickens [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 759-770.
[6]	ZHAO Wenqing, BO Xinwen, PU Na, ZHANG Yuxia, CHEN Xuke, ZHANG Yanyan, SUN Yan, QI Meng, WANG Zhengrong. Bioinformatics Analysis and Prokaryotic Expression of EgBAL Protein of Echinococcus granulosus [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 801-810.
[7]	ZHOU Fangting. Cloning,Molecular Characteristics and Tissue Differential Expression Analysis of KLF15 Gene in Buffalo [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3715-3725.
[8]	LI Feng, XIE Bin, ZHANG Shuai. Isolation，Identification and Genome Sequence Analysis of Two Recombinant Strains of Avian Infectious Bronchitis Virus [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3958-3969.
[9]	WU Jingnan, LI Zhanhong, LI Fuxiang, ZHANG Zhenxing, ZHANG Yifang, SONG Jianling. Isolation,Identification and Biological Characterization Analysis of Phage BP32 from Multidrug-Resistant Escherichia coli [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3540-3551.
[10]	ZHANG Xuedi, XU Kai, YIN Shuo, LIU Jing, WANG Zhonghao, QIN Jianhua, WU Pengyuan, WANG Chuanwen. Cloning,Sequence Analysis and Expression Characteristics of Carboxylesterase 2 Gene in Dermanyssus gallinae [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7): 2973-2983.
[11]	QIU Jicheng, YANG Yuxin, ZHANG Lu, WEN Zeyu, CHEN Sumeng, CAO Xingyuan. Application of Mass Spectrometry Techniques in the Characterization and Quantitative Analysis of Therapeutic Protein Drugs in Veterinarian [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2319-2329.
[12]	YU Fuxin, WU Siyu, YU Jieshi, ZHANG Xiaoai, YU Ting, DONG Bo, LI Jianbo, WANG Lei, WEI Wenkang. Isolation,Identification,Whole Genome Sequencing and Genetic Evolution Analysis of Goose Astrovirus in Some Areas of Guangdong Province [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2633-2643.
[13]	HUANG Liqing, DUAN Anqin, ZHENG Haiying, YANG Chunyan, SHANG Jianghua. Sequence Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of SFRP1 Gene in Buffalo [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 1807-1818.
[14]	HOU Gongmingzhu, ZHOU Haiqin, YU Xingyu, LI Nana, LI Yang, QU Yonggang, LI Yanfang, LIANG Yan, YAN Duo, YANG Shuhan, QIU Zhengqing, CHEN Siqi. Biological Characteristics and Whole Genome Analysis of a Multidrug-resistant Klebsiella pneumoniae Phage of Sheep Origin [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 2047-2057.
[15]	HUANG Shilei, LUO Gan, CHENG Peng, NIU Zheng, GUO Jianhua, ZOU Hong. Epidemic Report of Classical Swine Fever Virus in Pigs from 2005 to 2023 of China:Meta-Analysis and Systematic Review [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 2058-2070.

Molecular Characteristics Analysis of Two Strains of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments