鹅FoxO1基因cDNA序列的克隆及组织表达

Abstract

Abstract: In order to study the function of goose forkhead box O1 (FoxO1) gene, in this study, according to the highly conservative region of FoxO1 gene sequences from chicken (Gallus gallus)，human (Homo sapiens)，and mouse (Mus musculus)in the GenBank，the specific primers were designed to clone the CDS region of goose FoxO1 gene using the RT-PCR method，and the bioinformatics analyses of FoxO1 were conducted.The results showed that the full length of CDS region of FoxO1 gene was obtained, and the similarities of nucleotide sequence of goose with chicken, human and mouse were 97%, 83%, 82%, respectively. The expression level of FoxO1 gene in various tissues of Sichuan White goose showed: sebum> abdominal fat > crureus > pectorales > liver. In conclusion, FoxO1 gene could be expressed in several tissues, and showed an evident expression difference.

Key words: Sichuan White goose; forkhead box O1 gene; sequence analysis; tissue expression

YANG Lu, WEI Shou-hai. Cloning and Tissues Expression Analysis of Goose Forkhead Box O1 (FoxO1) Gene cDNA Sequence[J]. .

References

1 朱宇旌,于治姣,张勇,等. FoxO转录因子的活性调控及其对骨骼肌生长发育的调节［J］. 动物营养学报,2013,25(4): 677～684.
2 陈小玲,黄志清,毛湘冰,等. FoxO1的功能研究进展［J］.中国畜牧兽医,2011,38(9): 90～93.
3 张辉,史新娥,袁媛,等.FoxO1抑制猪骨骼肌MyHCⅠ的表达［J］.中国生物化学与分子生物学报,2010,26(3):283～289.
4 张野,崔世泉,狄生伟,等. 叉头框转录因子Ｏ亚族１基因对猪肾周脂肪沉积代谢的影响［J］. 中国畜牧兽医,2013,40(2):117～120.
5 杨燕军,白亮,庞卫军,等. 猪FoxO1基因cDNA部分序列的克隆及其组织表达［J］. 畜牧兽医学报,2007,38(11):1143～1148.
6 杨燕军,庞卫军,白亮,等. 八眉猪、长白猪和长×八杂交猪肌肉组织中FoxO1基因的表达［J］. 遗传,2008,30(2):185～189.
7 罗明娟.热卡限制对高脂饲养大鼠肝脏糖异生有关基因表达的影响［D］.武汉：华中科技大学,2008.
8 Allen D L, Unterman T G. Regulation of myostatin expression and myoblast differentiation by FoxO1 and SMAD transcription factors ［J］. American Journal of Physiology-cell Physiology, 2007, 292(1): C188～C199.
9 Arden K C, Biggs W H. Regulation of the FoxO family of transcription factors by phosphatidylinositol-3 kinase-ac-tivated signaling ［J］. Archives of Biochemistry and Biophysics, 2002, 403(2): 292～298.
10 Bois P R J, Brochard V F, Salin-Cantegrel A V A, et al. FoxO1a-cyclic GMP-dependent kinase Ⅰ interactions orchestrate myoblast fusion ［J］. Molecular and Cellular Biochemistry, 2005, 25: 7645～7656.
11 Brunet A, Sweeney L B, Sturgill J F, et al. Stress-dependent regulation of FoxO tanscription factors by the SIRT1 deacetylase［J］. Science, 2004, 303(5666): 2011～2015.
12 Czech M P. Insulin’s expanding control of fork heads ［J］. PNAS, 2003, 100(20): 11198～11200.
13 Farmer S R. The forkhead transcription factor FoxO1: A possible link between obesity and insulin resistance ［J］. Molecular Cell, 2003, 11: 6～8.
14 Hribal M L, Nakae J, Kitamura T, et al. Regulation of insulin-like growth factor-dependent myoblast differentiation by FoxO forkhead transcription factors ［J］. Journal of Cellular Biochemistry, 2003, 162(4): 535～541.
15 Kaestner K H, Knchel W, Martinez D E. Unified nomen-clature for the winged helix/forkhead transcription factors ［J］. Genes & Development, 2000, 14(2): 142～146.
16 Kamei Y, Miura S, Suzuki M, et al. Skeletal muscle FoxO1 (FKHR)-transgenic mice have less skeletal muscle mass, down-regulated type Ⅰ (slow twitch/red muscle) fiber genes, and impaired glycemic control ［J］. Journal of Biological Chemistry, 2004, 279: 41114～41123.
17 Kim H J, Kobayashi M, Sasaki T, et al. Overexpression of FoxO1 in the hypothalamus and pancreas causes obesity and glucose intolerance ［J］. Journal of Endocrinology, 2012, 153(2): 659～671.
18 McFarlane C, Plummer E, Thomas M, et al. Myostatin induces cachexia by activating the ubiquitin proteolytic system through an NF-kappaB-independent, FoxO1-dependent mechanism ［J］. Journal of Cellular Physiology, 2006, 209(2): 501～514.
19 Nakae J, Kitamura T, Kitamura Y, et al. The forkhead transcription factor FoxO1 regulates adipocyte differentiation ［J］. Developmental Cell, 2003, 4(1): 119～129.
20 Puig O, Marr M T, Ruhf M L, et al. Control of cell number by drosophila FoxO: Downstream and feedback regulation of the insulin receptor pathway ［J］. Genes & Development, 2003, 17(16): 2006～2020.
21 Siriett V, Nicholas G, Berry C, et al. Myostatin negatively regulates the expression of the steroid receptor co-factor ARA70［J］. Journal of Cellular Physiology, 2006, 206(1): 255～263.
22 Smith I J，Alamdari N，O’neal P，et al. Sepsis increases the expression and activity of the transcription factor forkhead box O1 (FoxO1) in skeletal muscle by a glucocorticoid dependent mechanism ［J］. International Journal of Biochemistry and Cell Biology, 2010,42(5): 701～711.

[1]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[2]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[3]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[4]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[5]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[6]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[7]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[8]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[9]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[10]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.
[11]	GUO Mengjiao, LI Mingtao, TAN Huihui, ZHANG Chengcheng, ZHANG Xiaorong, WU Yantao. Study on Cloning and Expression Characteristic of NLRC3 Gene in Rabbit [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3940-3949.
[12]	WANG Zhengfan, ZHU Lisai, LI Fei, XIANG Rui, LI Yiyun, YING Biyun, WANG Guiping, BAI Aiquan, JIA Aiqing. Pathogenicity and Whole Genome Sequence Analysis of Porcine Deltacoronavirus CH7328 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4143-4153.
[13]	YIN Dejing, ZHANG Yundan, YUE Jun, LI Qiaobin, HE Ling, LI Tao, WANG Kaigong, CHENG Zhentao. Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 44-54.
[14]	PAN Pengcheng, WEN Binhua, XIE Wan, JIANG Changjin, JIAO Di, CHEN Baojian, GUAN Zhihui, XIE Bingkun. Sequence Analysis,Eukaryotic Expression Vector Construction and Tissue Expression Analysis of PDK4 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2337-2347.
[15]	QIN Benyuan, YANG Shuai, ZHANG Yanwei, LI Wenxin, LIU Hong, WU Yiqi, ZHANG Xuelian, LI Wenxia, YANG Yang, CAI Chunbo, GAO Pengfei, GUO Xiaohong, LI Bugao, CAO Guoqing. Study on Cloning,Bioinformatics Analysis and Expression Profile of ASB2 Gene CDS in Pig [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1289-1298.

Cloning and Tissues Expression Analysis of Goose Forkhead Box O1 (FoxO1) Gene cDNA Sequence

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments