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Isolation, Identification and Antibiotics Susceptibility of Mannheimia haemolytica from Sheep Lungs

FENG Xu-fei1, DAO Xiao-fang1, WANG Zhi-min1, TANG Cheng1, LI Ding-fei2, YANG Fa-long1   

  1. 1.College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;2.Chengdu Aquatic Animal Inspection and Quarantine, Chengdu 610071, China
  • Revised:2014-05-20 Online:2014-08-20 Published:2014-08-22

Abstract: The aim of this study was to isolate the etiological bacteria of sheep pneumoniae and evaluate the antibiotic susceptibility of the isolates. Bacteria was isolated from lungs of sick sheep, and further identified with bio-chemical tests and PCR method, the antibiotic susceptibility of the isolates was determined. The results showed that the isolates were gram negative, organisms with haemolytica. Bio-chemical and PCR method proved that the isolates were Mannheimia haemolytica. The isolates were susceptible to most of the tested antibiotics such as enrofloxacin, gentamicin and tetracycline. The results obtained in this study provided useful information for treatment of Mannheimia haemolytica infection.

Key words: sheep; Mannheimia haemolytica; identification; antibiotic resistance